Association of connexin36 with adherens junctions at mixed synapses and distinguishing electrophysiological features of those at mossy fiber terminals in rat ventral hippocampus.

Deepthi Thomas, Antonia Recabal-Beyer, Joanne Mm Senecal, Demitre Serletis, Bruce D Lynn, Michael F Jackson, James I Nagy
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Abstract

Background: Granule cells in the hippocampus project axons to hippocampal CA3 pyramidal cells where they form large mossy fiber terminals. We have reported that these terminals contain the gap junction protein connexin36 (Cx36) specifically in the stratum lucidum of rat ventral hippocampus, thus creating morphologically mixed synapses that have the potential for dual chemical/electrical transmission.

Methodology: Here, we used various approaches to characterize molecular and electrophysiological relationships between the Cx36-containing gap junctions at mossy fiber terminals and their postsynaptic elements and to examine molecular relationships at mixed synapses in the brainstem.

Results: In rat and human ventral hippocampus, many of these terminals, identified by their selective expression of vesicular zinc transporter-3 (ZnT3), displayed multiple, immunofluorescent Cx36-puncta representing gap junctions, which were absent at mossy fiber terminals in the dorsal hippocampus. In rat, these were found in close proximity to the protein constituents of adherens junctions (i.e., N-cadherin and nectin-1) that are structural hallmarks of mossy fiber terminals, linking these terminals to the dendritic shafts of CA3 pyramidal cells, thus indicating the loci of gap junctions at these contacts. Cx36-puncta were also associated with adherens junctions at mixed synapses in the brainstem, supporting emerging views of the structural organization of the adherens junction-neuronal gap junction complex. Electrophysiologically induced long-term potentiation (LTP) of field responses evoked by mossy fiber stimulation was greater in the ventral than dorsal hippocampus.

Conclusions: The electrical component of transmission at mossy fiber terminals may contribute to enhanced LTP responses in the ventral hippocampus.

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大鼠腹侧海马混合突触中连接蛋白36与粘连接头的关联以及苔藓纤维末端的电生理特征的区分。
背景:海马颗粒细胞的轴突投射到海马CA3锥体细胞,在那里形成大的苔藓纤维终端。方法:在此,我们采用各种方法描述苔藓纤维末端含 Cx36 的缝隙连接及其突触后元件之间的分子和电生理关系,并研究脑干混合突触的分子关系:结果:在大鼠和人的腹侧海马中,许多通过选择性表达囊泡锌转运体-3(ZnT3)而被识别的末端显示了多个免疫荧光Cx36-puncta代表的间隙连接,这些间隙连接在海马背侧的苔藓纤维末端是不存在的。在大鼠体内,这些结点与苔藓纤维末端的结构标志--粘连接头的蛋白质成分(即 N-cadherin和 nectin-1)非常接近,它们将苔藓纤维末端与 CA3 锥体细胞的树突轴连接起来,从而表明了这些接触点上的间隙连接位置。在脑干的混合突触中,Cx36-结点也与粘连接头相关,这支持了新出现的粘连接头-神经元间隙连接复合体结构组织的观点。苔藓纤维刺激诱发的场反应的电生理诱导长期电位(LTP)在海马腹侧比背侧更强:结论:苔藓纤维末端传输的电成分可能有助于增强腹侧海马的 LTP 反应。
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