Maud Billaud, Ilias Theodorou, Quentin Lamy-Besnier, Shiraz A. Shah, François Lecointe, Luisa De Sordi, Marianne De Paepe, Marie-Agnès Petit
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引用次数: 0
Abstract
Bulk microbiome, as well as virome-enriched shotgun sequencing only reveals the double-stranded DNA (dsDNA) content of a given sample, unless specific treatments are applied. However, genomes of viruses often consist of a circular single-stranded DNA (ssDNA) molecule. Pre-treatment and amplification of DNA using the multiple displacement amplifica-tion (MDA) method enables conversion of ssDNA to dsDNA, but this process can lead to over-representation of these circular ssDNA genomes. A more recent alternative permits to bypass the amplification
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