Viral aptamer screening and aptamer-based biosensors for virus detection: A review

IF 4.4 2区化学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY ACS Applied Polymer Materials Pub Date : 2024-07-17 DOI:10.1016/j.ijbiomac.2024.133935

Changchun Hu , Shuting Yang , Shuo Li , Xueying Liu , Yuan Liu , Zhu Chen , Hui Chen , Song Li , Nongyue He , Haipo Cui , Yan Deng

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Abstract

Virus-induced infectious diseases have a detrimental effect on public health and exert significant influence on the global economy. Therefore, the rapid and accurate detection of viruses is crucial for effectively preventing and diagnosing infections. Aptamer-based detection technologies have attracted researchers' attention as promising solutions. Aptamers, small single-stranded DNA or RNA screened via systematic evolution of ligands by exponential enrichment (SELEX), possess a high affinity towards their target molecules. Numerous aptamers targeting viral marker proteins or virions have been developed and widely employed in aptamer-based biosensors (aptasensor) for virus detection. This review introduces SELEX schemes for screening aptamers and discusses distinctive SELEX strategies designed explicitly for viral targets. Furthermore, recent advances in aptamer-based biosensing methods for detecting common viruses using different virus-specific aptamers are summarized. Finally, limitations and prospects associated with developing of aptamer-based biosensors are discussed.

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用于病毒检测的病毒适配体筛选和基于适配体的生物传感器：综述。

由病毒引起的传染病对公众健康造成有害影响，并对全球经济产生重大影响。因此，快速准确地检测病毒对于有效预防和诊断感染至关重要。基于色聚体的检测技术作为一种前景广阔的解决方案引起了研究人员的关注。适配体是通过指数富集配体的系统进化（SELEX）筛选出的小单链 DNA 或 RNA，对目标分子具有高亲和力。目前已开发出大量针对病毒标记蛋白或病毒的适配体，并广泛应用于基于适配体的病毒检测生物传感器（aptasensor）中。本综述介绍了筛选适配体的 SELEX 方案，并讨论了专门针对病毒靶标设计的独特 SELEX 策略。此外，还总结了使用不同的病毒特异性适配体检测常见病毒的基于适配体的生物传感方法的最新进展。最后，还讨论了开发基于适配体的生物传感器的局限性和前景。

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来源期刊

ACS Applied Polymer Materials Multiple-

CiteScore

7.20

自引率

6.00%

发文量

810

期刊介绍： ACS Applied Polymer Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics, and biology relevant to applications of polymers. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates fundamental knowledge in the areas of materials, engineering, physics, bioscience, polymer science and chemistry into important polymer applications. The journal is specifically interested in work that addresses relationships among structure, processing, morphology, chemistry, properties, and function as well as work that provide insights into mechanisms critical to the performance of the polymer for applications.