{"title":"Patterned microarrays or nanoscale assemblies for optical sensing of biomarkers","authors":"","doi":"10.1016/j.trac.2024.117873","DOIUrl":null,"url":null,"abstract":"<div><p>Array-based micro- or nanoscale substrates are particularly attractive for biomolecular sensing due to the periodicity of the engineered nanostructures that provide impressively enhanced and spatially uniform optical outputs. However, the complexity in the manufacturing of nanoscale substrates and laborious data processing restrict their real-world monitoring uses. This review will summarize the fabrications of state-of-the-art microarray or nanoassembly technology, the methodologies in biomarker recognition and quantification, and applications in biological sensing and diagnosis of various diseases. As the primary applications in clinical diagnosis, the optical monitoring of microRNAs, proteins and other small-molecule biomarkers will be reviewed according to the specific optical transducing mechanisms and biomolecular interactions involved. Choices of the optical signal acquisition, such as the bulk measurement-based analysis or digital counting will be elaborated discussed. Finally, the remaining challenges and perspectives in the design of the next generation of patterned microarrays or nanoassemblies for sensing purposes will be highlighted.</p></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":11.8000,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Analytical Chemistry","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S016599362400356X","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Array-based micro- or nanoscale substrates are particularly attractive for biomolecular sensing due to the periodicity of the engineered nanostructures that provide impressively enhanced and spatially uniform optical outputs. However, the complexity in the manufacturing of nanoscale substrates and laborious data processing restrict their real-world monitoring uses. This review will summarize the fabrications of state-of-the-art microarray or nanoassembly technology, the methodologies in biomarker recognition and quantification, and applications in biological sensing and diagnosis of various diseases. As the primary applications in clinical diagnosis, the optical monitoring of microRNAs, proteins and other small-molecule biomarkers will be reviewed according to the specific optical transducing mechanisms and biomolecular interactions involved. Choices of the optical signal acquisition, such as the bulk measurement-based analysis or digital counting will be elaborated discussed. Finally, the remaining challenges and perspectives in the design of the next generation of patterned microarrays or nanoassemblies for sensing purposes will be highlighted.
期刊介绍:
TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.