Mammaliicoccus sciuri's Pan-Immune System and the Dynamics of Horizontal Gene Transfer Among Staphylococcaceae: a One-Health CRISPR Tale.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-07-22 DOI:10.1007/s12275-024-00156-7
Allan de Carvalho, Marcia Giambiagi-deMarval, Ciro César Rossi
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Abstract

Recently emancipated from the Staphylococcus genus due to genomic differences, Mammaliicoccus sciuri, previously classified as an occasional pathogen, emerges as a significant player in the landscape of resistance gene dissemination among Staphylococcaceae. Despite its classification, its role remained enigmatic. In this study, we delved into the genomic repertoire of M. sciuri to unravel its contribution to resistance and virulence gene transfer in the context of One Health. Through comprehensive analysis of publicly available genomes, we unveiled a diverse pan-immune system adept at defending against exogenous genetic elements, yet concurrently fostering horizontal gene transfer (HGT). Specifically, exploration of CRISPR-Cas systems, with spacer sequences as molecular signatures, elucidated a global dissemination pattern spanning environmental, animal, and human hosts. Notably, we identified the integration of CRISPR-Cas systems within SCCmecs (Staphylococcal Cassette Chromosome mec), harboring key genes associated with pathogenicity and resistance, especially the methicillin resistance gene mecA, suggesting a strategic adaptation to outcompete other mobile genetic elements. Our findings underscored M. sciuri's active engagement in HGT dynamics and evolutionary trajectories within Staphylococcaceae, emphasizing its central role in shaping microbial communities and highlighting the significance of understanding its implications in the One Health framework, an interdisciplinary approach that recognizes the interconnectedness of human, animal, and environmental health to address global health challenges.

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Mammaliicoccus sciuri 的泛免疫系统与葡萄球菌之间的水平基因转移动态:一个健康 CRISPR 故事。
由于基因组差异,最近从葡萄球菌属中分离出来的 Mammaliicoccus sciuri 以前被归类为偶发性病原体,现在则成为葡萄球菌科耐药基因传播领域的重要角色。尽管已被归类,但它的作用仍是个谜。在本研究中,我们深入研究了M. sciuri的基因组,以揭示其在 "一体健康 "背景下对抗性和毒力基因转移的贡献。通过对公开的基因组进行全面分析,我们揭示了一个多样化的泛免疫系统,该系统善于抵御外源遗传因子,但同时也促进了水平基因转移(HGT)。具体来说,我们以CRISPR-Cas系统的间隔序列为分子特征,阐明了一种跨越环境、动物和人类宿主的全球传播模式。值得注意的是,我们发现了CRISPR-Cas系统在SCCmecs(葡萄球菌盒式染色体mec)中的整合,该系统携带与致病性和抗性相关的关键基因,特别是甲氧西林抗性基因mecA,这表明该系统具有战略适应性,能与其他移动遗传因子竞争。我们的发现强调了M. sciuri积极参与葡萄球菌科内的HGT动态和进化轨迹,强调了它在塑造微生物群落中的核心作用,并突出了了解其在 "一个健康 "框架中的意义,该框架是一种跨学科方法,承认人类、动物和环境健康的相互关联性,以应对全球健康挑战。
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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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