A Novel Method for the Assessment of Feeding Rate as a Phenotypic Endpoint for the Impact of Pollutants in Daphnids

IF 3.6 4区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Environmental Toxicology and Chemistry Pub Date : 2024-07-26 DOI:10.1002/etc.5960
Emma Rowan, Anne Leung, Konstantinos Grintzalis
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Abstract

Traditional approaches for monitoring aquatic pollution primarily rely on chemical analysis and the detection of pollutants in the aqueous environments. However, these methods lack realism and mechanistic insight and, thus, are increasingly supported by effect-based methods, which offer sensitive endpoints. In this context, daphnids, a freshwater species used extensively in molecular ecotoxicology, offer fast and noninvasive approaches to assess the impact of pollutants. Among the phenotypic endpoints used, feeding rate is a highly sensitive approach because it provides evidence of physiological alterations even in sublethal concentrations. However, there has been no standardized method for measuring feeding rate in daphnids, and several approaches follow different protocols. There is a diversity among tests employing large volumes, extensive incubation times, and high animal densities, which in turn utilize measurements of algae via fluorescence, radiolabeling, or counting ingested cells. These tests are challenging and laborious and sometimes require cumbersome instrumentation. In the present study, we optimized the conditions of a miniaturized fast, sensitive, and high-throughput assay to assess the feeding rate based on the ingestion of fluorescent microparticles. The protocol was optimized in neonates in relation to the concentration of microplastic and the number of animals to increase reproducibility. Daphnids, following exposures to nonlethal concentrations, were incubated with microplastics; and, as filter feeders, they ingest microparticles. The new approach revealed differences in the physiology of daphnids in concentrations below the toxicity limits for a range of pollutants of different modes of action, thus proving feeding to be a more sensitive and noninvasive endpoint in pollution assessment. Environ Toxicol Chem 2024;43:2211–2221. © 2024 The Author(s). Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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将摄食率作为水蚤受污染物影响的表型终点的新型评估方法
传统的水生污染监测方法主要依赖于化学分析和水环境中污染物的检测。然而,这些方法缺乏现实性和机理洞察力,因此越来越多地采用基于效应的方法来提供敏感终点。在这种情况下,在分子生态毒理学中广泛使用的淡水物种--水蚤,为评估污染物的影响提供了快速、非侵入性的方法。在使用的表型终点中,摄食率是一种高度敏感的方法,因为它甚至可以提供亚致死浓度下生理变化的证据。然而,目前还没有测量水蚤摄食率的标准化方法,有几种方法遵循不同的规程。不同的测试方法采用了大容量、长孵化时间和高动物密度,进而通过荧光、放射性标记或计算摄取的细胞来测量藻类。这些测试既具有挑战性又费力,有时还需要笨重的仪器。在本研究中,我们优化了一种小型化的快速、灵敏和高通量检测方法的条件,该方法可根据荧光微颗粒的摄取来评估摄食率。为了提高重现性,我们根据微塑料的浓度和动物的数量对新生儿的实验方案进行了优化。水蚤在接触非致死浓度的微塑料后,与微塑料一起孵化;作为滤食动物,它们会摄取微颗粒。这种新方法揭示了水蚤在不同作用模式的污染物浓度低于毒性限值时的生理差异,从而证明摄食是污染评估中更为敏感和非侵入性的终点。环境毒物化学 2024;00:1-11。© 2024 The Author(s).环境毒理学与化学》由 Wiley Periodicals LLC 代表 SETAC 出版。
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来源期刊
CiteScore
7.40
自引率
9.80%
发文量
265
审稿时长
3.4 months
期刊介绍: The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.
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Correction. Spotlights are papers selected by editors published in peer-reviewed journals that may be more regionally specific or appearing in languages other than English Issue Information - Cover Editorial Board and Table of Contents Detection and Prediction of Toxic Aluminum Concentrations in High-Priority Salmon Rivers in Nova Scotia.
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