Isolation and biochemical screening of Phosphate Solubilizing Bacteria from Kans grass rhizosphere of Fly Ash Dump Sites near NTPC, Angul

S. Padhi, Karubakee Dhal, B. S. Sipra, B. Pati
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Abstract

Soil bacteria, capable of converting insoluble phosphorus into soluble, plant-accessible forms, collectively known as phosphate-solubilizing bacteria (PSB), have emerged as an eco-friendly and cost-effective solution for supplying phosphorus to plants. This study presents preliminary insights into the rhizospheric bacterial population associated with the vigorously growing kans grass (Saccharum spontaneum L.) at different fly ash disposal sites of NTPC, Kaniha in Angul district, Odisha. In the quest for efficient plant growth promoting rhizobacteria (PGPR) strains with diverse beneficial activities, a total of sixteen bacterial isolates were collected from the rhizosphere of chosen plant species and identified, with only ten isolates underwent biochemical characterization. Among these, four isolates exhibiting a higher phosphate-solubilizing index were further characterized, serving as a foundation for future exploration of the bio-prospective potential of rhizosphere bacteria in this unique ecological niche. Further assessment of these isolates, showcasing several plant growth-promoting (PGP) traits, is essential to determine their efficacy as potent PGPR agents.
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安古尔邦 NTPC 附近粉煤灰倾倒场甘草根瘤中磷酸盐溶解细菌的分离和生化筛选
土壤细菌能够将不溶解的磷转化为植物可溶解的形式,统称为磷酸盐溶解细菌(PSB),已成为向植物提供磷的一种生态友好且具有成本效益的解决方案。本研究对奥迪沙邦安古尔区卡尼哈的 NTPC 公司不同粉煤灰处理场中与生长旺盛的坎斯草(Saccharum spontaneum L.)相关的根瘤菌群进行了初步研究。为了寻找具有多种有益活性的高效植物生长促进根瘤菌(PGPR)菌株,我们从所选植物物种的根瘤层中共收集了 16 个细菌分离物并进行了鉴定,其中只有 10 个分离物进行了生化鉴定。在这些分离物中,有四个分离物表现出较高的磷酸盐溶解指数,这为今后探索根瘤菌在这一独特生态位中的生物前景潜力奠定了基础。这些分离物具有多种植物生长促进(PGP)特性,对它们的进一步评估对于确定其作为强效 PGPR 制剂的功效至关重要。
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