Gas chromatography-mass spectrometry and acute toxicity studies of Annona muricata Ethanol stem bark extract

Abstract

Plants with therapeutic potentials are considered to be the main source of phytoconstituents that can be used for the treatment of various ailments. This work focuses on determining the acute toxicity and the phytochemical constituents present in ethanol stem bark extracts of Annona muricata using Gas chromatography-mass spectroscopy. Lorke's toxicity testing method was used to determine the acute toxicity of the A. muricata stem bark extract. The result of the quantitative phytochemical GC-MS analysis of the stem bark showed that the ethanol stem bark extracts contained 13 compounds at different retention times. The dominant constituents present in the stem bark ethanol extracts of A. muricata were; Diisooctyl phthalate (25.81%), 1,2,3-Benzenetriol (14.41%),Phthalic acid (12.47%), Silane (7.60%) and 2-Heptanol (7.01) while the other constituents have less than 6% abundance respectively. Studies on acute toxicity revealed that the LD50 was > 5000 mg/kg of body weight.