Effects of an Chinese Herbal Compound Prescription on Simulated Transport Stress in Mice

Abstract

Background: In order to alleviate the transport stress reaction of animals, three kinds of chinese herbs with sedative and tranquilizing properties were extracted with ethanol and proportionally composed into a chinese herbal compound prescription. In addition, isorhynchophylline in leaves of Uncaria rhynchophylla was isolated and purified for use. Methods: The enzyme-linked Immunosorbent assay (ELISA) was used to measure the changes in serum stress indicators and hormone levels, three classification blood cell counter and automatic biochemistry analyser was used to measure blood indicators and other biochemical indicators of each group mice before and after transport stress. Result: The results showed that the blood physiological indexes such as blood glucose (GLU) level, biochemical indexes such as lactate dehydrogenase (LDH) and hormone levels such as adrocorticotropic hormone (ACTH) and other stress indexes were significantly higher in mice after transport than before. The compound agents and isorhynchophylline played a role in regulating the stress indices of mice after the simulating transport processs and have the effect of significantly alleviating the transport stress response in mice, with a view to provide reference for alleviating the adverse effects of transport stress on the animal husbandry industry.