Management of Corneal Ulcers using Autologous Serum, Platelet Rich Plasma and Processed Human Amniotic Membrane in Dogs

Abstract

Background: In dogs the corneal ulcers are one of the major ophthalmic emergencies and can cure with routine medications, but in delay presentation will become challenge for veterinarians. In such cases advanced therapeutic management such as serum, platelet reach plasma and corneal grafts are needed for favorable outcome. Methods: The present study was conducted on 24 dogs (29 eyes) with corneal ulcer categorized as a superficial (Group-I, n=15), deep corneal ulcers (Group-II, n=8) and descemetocele (Group-III, n=6) which were treated with autologous serum, Platelet Rich Plasma and Processed decellularized human amniotic membrane grafting, respectively. All the affected eyes were given routine topical medications with eye drops Moxifloxacin, Flurbiprofen q.i.d till healing, 1% Atropine sulphate b.i.d for 5 days and Oral Doxycycline was given @ 5 mg/kg b.i.d for 10 days. Result: The group-I (n=11, 15 eyes) treated with autologous serum eight to ten times-a-day as per severity with routine medication. Two dogs with entropion which was corrected first. The eyes gained vision within 10th-15th days. Developed complications managed medicinally till complete recovery. The group-II (n=7, 8 eyes) treated with platelet rich plasma drops five to seven times-a-day with routine medication. 75% eyes recovered up to 45th day. Developed complication were managed medicinally till complete recovery. The group-III (n=5, 6 eyes) treated with Decellularized Processed Human Amniotic Membrane Graft. 4 eyes gained complete transparency on 28th day of therapy while in 2 eyes developed complication were managed medicinally.