Epidemiology and molecular characterization of Mycobacterium tuberculosis including a drug-resistant strain associated with mortality of Asian elephants in Nepal 2019–2022

IF 2.8 3区医学 Q3 IMMUNOLOGY Tuberculosis Pub Date : 2024-07-26 DOI:10.1016/j.tube.2024.102550

Arjun Pandit , Jeewan Thapa , Amir Sadaula , Yasuhiko Suzuki , Chie Nakajima , Susan K. Mikota , Naresh Subedi , Bijaya Kumar Shrestha , Michito Shimozuru , Bhawana Shrestha , Bijendra Raya , Sanjay Chaudhary , Sarad Paudel , Toshio Tsubota

{"title":"Epidemiology and molecular characterization of Mycobacterium tuberculosis including a drug-resistant strain associated with mortality of Asian elephants in Nepal 2019–2022","authors":"Arjun Pandit , Jeewan Thapa , Amir Sadaula , Yasuhiko Suzuki , Chie Nakajima , Susan K. Mikota , Naresh Subedi , Bijaya Kumar Shrestha , Michito Shimozuru , Bhawana Shrestha , Bijendra Raya , Sanjay Chaudhary , Sarad Paudel , Toshio Tsubota","doi":"10.1016/j.tube.2024.102550","DOIUrl":null,"url":null,"abstract":"<div>Tuberculosis (TB) is an emerging threat to the survival of elephants in Nepal. We investigated the lung tissue samples from nine elephants that died from 2019 to 2022 in Nepal using culture, conventional PCR, and loop-mediated isothermal amplification (LAMP) and then performed genotyping of five PCR-positive isolates to understand the possible transmission dynamics of Mycobacterium tuberculosis (Mtb). Results showed that two-thirds (6/9) of elephants were confirmed to be infected from Mtb by LAMP, 5/9 by PCR, and 4/9 by culture. Genotyping of Mtb isolates showed that elephants were infected with the Indo-Oceanic and Beijing lineages including an isoniazid-resistant Beijing lineage. MIRU-VNTR-based phylogeny, gyrA, and katG sequencing showed the possibility of ongoing transmission of Indo-Oceanic lineages and likely transmission of the drug-resistant Beijing lineage from human to elephant. Implementation of comprehensive surveillance and preventive measures are urgently needed to address this zoonotic disease and protect elephants from TB in Nepal.</div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102550"},"PeriodicalIF":2.8000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tuberculosis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1472979224000763","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Tuberculosis (TB) is an emerging threat to the survival of elephants in Nepal. We investigated the lung tissue samples from nine elephants that died from 2019 to 2022 in Nepal using culture, conventional PCR, and loop-mediated isothermal amplification (LAMP) and then performed genotyping of five PCR-positive isolates to understand the possible transmission dynamics of Mycobacterium tuberculosis (Mtb). Results showed that two-thirds (6/9) of elephants were confirmed to be infected from Mtb by LAMP, 5/9 by PCR, and 4/9 by culture. Genotyping of Mtb isolates showed that elephants were infected with the Indo-Oceanic and Beijing lineages including an isoniazid-resistant Beijing lineage. MIRU-VNTR-based phylogeny, gyrA, and katG sequencing showed the possibility of ongoing transmission of Indo-Oceanic lineages and likely transmission of the drug-resistant Beijing lineage from human to elephant. Implementation of comprehensive surveillance and preventive measures are urgently needed to address this zoonotic disease and protect elephants from TB in Nepal.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

2019-2022 年尼泊尔亚洲象结核分枝杆菌的流行病学和分子特征，包括与亚洲象死亡有关的耐药菌株。

结核病（TB）是尼泊尔大象生存面临的新威胁。我们使用培养、传统 PCR 和环介导等温扩增（LAMP）技术调查了 2019 年至 2022 年期间在尼泊尔死亡的 9 头大象的肺组织样本，然后对 5 个 PCR 阳性分离物进行了基因分型，以了解结核分枝杆菌（Mtb）可能的传播动态。结果显示，三分之二（6/9）的大象通过 LAMP、5/9 通过 PCR 和 4/9 通过培养证实感染了 Mtb。Mtb 分离物的基因分型显示，大象感染了印度洋系和北京系，其中包括耐异烟肼的北京系。基于 MIRU-VNTR 的系统发育、gyrA 和 katG 测序表明，印度洋系有可能继续传播，耐药北京系也有可能从人类传播给大象。尼泊尔急需采取全面的监测和预防措施来应对这种人畜共患病，保护大象免受结核病的危害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.