Investigating the altered expression of miR-486-5p and miR-novel-chr1_40444 in dysglycemia in a South African population

IF 3.1 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Journal of Diabetes Investigation Pub Date : 2024-08-01 DOI:10.1111/jdi.14278

Cecil J Weale, Chanelle Schroeder, Don M Matshazi, Saarah FG Davids, Rajiv T Erasmus, Andre P Kengne, Glenda M Davison, Tandi E Matsha

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Abstract

Aims

This study aims to investigate miR-486-5p and miR-novel-chr1_40444 expressions in dysglycemic individuals. Validating RNA-sequencing findings in a larger sample via reverse transcription qPCR (RT-qPCR), we aim to address global diagnostic and screening limitations, using an African cohort as an example.

Materials and Methods

This cross-sectional study involved 1,271 individuals [normoglycemic (n = 974), prediabetic (n = 206), screen-detected type 2 diabetes (n = 91)] from the ongoing Vascular and Metabolic Health (VMH) study in Cape Town, South Africa. Whole blood miRNA expression was assessed using TaqMan-based RT-qPCR, with data normalized to an endogenous control (miR-16-5p).

Results

Significant underexpression was observed in prediabetes vs normoglycemia for miR-486-5p (P = 0.038), whilst both miRNAs demonstrated significant upregulation in screen-detected type 2 diabetes vs normoglycemia (miR-486-5p, P = 0.009; miR-novel-chr1_40444, P < 0.001), and screen-detected type 2 diabetes in comparison with prediabetes (miR-486-5p, P < 0.001; miR-novel-chr1_40444, P < 0.001). Multivariable regression analyses revealed pronounced interrelations between miR-novel-chr1_40444 and screen-detected type 2 diabetes in unadjusted and adjusted models (Model 1: P < 0.001, Model 2: P < 0.001, Model 3: P = 0.030). Moreover, receiver operating characteristic (ROC) curves revealed significantly enhanced diagnostic capabilities for screen-detected type 2 diabetes vs either normoglycemia (AUC = 0.971, P < 0.001), non-diabetes (AUC = 0.959, P < 0.001), or prediabetes (AUC = 0.902, P < 0.001) when combining the miRNAs with 2 h postprandial glucose.

Conclusions

This study demonstrated the enhanced power of incorporating miRNAs with traditional markers in distinguishing screen-detected type 2 diabetes, warranting further investigations on their unique role in the development of type 2 diabetes.

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研究南非人群中 miR-486-5p 和 miR-novel-chr1_40444 在血糖异常中的表达变化。

目的：本研究旨在调查血糖异常人群中 miR-486-5p 和 miR-novel-chr1_40444 的表达。通过反转录 qPCR（RT-qPCR）在更大样本中验证 RNA 序列研究结果，我们旨在以非洲队列为例，解决全球诊断和筛查的局限性：这项横断面研究涉及南非开普敦正在进行的血管和代谢健康（VMH）研究中的 1,271 人[血糖正常（n = 974）、糖尿病前期（n = 206）、筛查出的 2 型糖尿病（n = 91）]。使用基于 TaqMan 的 RT-qPCR 评估了全血 miRNA 表达，并将数据归一化为内源性对照（miR-16-5p）：结果：在糖尿病前期与正常血糖相比，miR-486-5p 的表达明显不足（P = 0.038），而在筛查出的 2 型糖尿病与正常血糖相比，这两种 miRNA 都出现了明显的上调（miR-486-5p，P = 0.009；miR-novel-chr1_40444，P 结论：这项研究证明，在糖尿病前期与正常血糖相比，miR-486-5p 的表达明显不足，而在筛查出的 2 型糖尿病与正常血糖相比，这两种 miRNA 都出现了明显的上调（miR-486-5p，P = 0.009；miR-novel-chr1_40444，P = 0.009）：这项研究表明，将 miRNA 与传统标记物结合在一起可增强区分筛查出的 2 型糖尿病的能力，因此有必要进一步研究 miRNA 在 2 型糖尿病发病过程中的独特作用。

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来源期刊

Journal of Diabetes Investigation ENDOCRINOLOGY & METABOLISM-

CiteScore

6.50

自引率

9.40%

发文量

218

审稿时长

6-12 weeks

期刊介绍： Journal of Diabetes Investigation is your core diabetes journal from Asia; the official journal of the Asian Association for the Study of Diabetes (AASD). The journal publishes original research, country reports, commentaries, reviews, mini-reviews, case reports, letters, as well as editorials and news. Embracing clinical and experimental research in diabetes and related areas, the Journal of Diabetes Investigation includes aspects of prevention, treatment, as well as molecular aspects and pathophysiology. Translational research focused on the exchange of ideas between clinicians and researchers is also welcome. Journal of Diabetes Investigation is indexed by Science Citation Index Expanded (SCIE).