Analysis of 12 kinds of cytokines in seminal plasma by flow cytometry and their correlations with routine semen parameters

IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Cytokine Pub Date : 2024-07-30 DOI:10.1016/j.cyto.2024.156718
Shan-Shan Tang, Jin-Chun Lu, Yan-Mei Ge, Yuan-Hua Xu, Xia Zhao, Yuan-Jiao Liang
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Abstract

Objective

To investigate the levels of 12 kinds of cytokines in seminal plasma and their correlations with routine semen parameters.

Methods

The remaining seminal plasma samples of 134 patients undergoing routine semen examination were collected for detecting cytokines. The parameters for sperm concentration, percentage of progressively motile sperm (PR), and motility were analyzed by a computer-assisted sperm analysis (CASA) system. According to the results of sperm concentration, PR and motility, 134 patients were divided into the normal routine semen parameters group, oligoasthenospermia group and azoospermia group. The levels of 12 kinds of cytokines in seminal plasma, including interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12P70, IL-17, interferin (IFN)-α, IFN-γ, and tumor necrosis factor (TNF)-α, were detected by flow cytometry. Two seminal plasma samples were detected for 10 times, respectively, to calculate the coefficients of variation (CV) of each cytokine. The linear range of each cytokine was measured using the standard, and the correlation coefficient (r) was calculated.

Results

The r2 of 12 kinds of cytokines detected by flow cytometry were all greater than 0.99. The reproducibility of 2 seminal plasma samples showed that the CVs of all cytokines were lower than 15 % except for TNF-α in sample 1 (15.15 %). Seminal plasma IL-6 levels were negatively correlated with semen volume (P < 0.01). Seminal plasma IL-5 levels were positively correlated with sperm concentration (P < 0.01). Seminal plasma IL-8 levels were negatively correlated with sperm motility (P < 0.01). Seminal plasma IL-8, IL-17 and IL-12P70 levels were negatively correlated with sperm PR (P < 0.05). In addition to the significant negative correlation between IL-5 and IL-17 (P < 0.05), there was a significant positive correlation between the majority of other cytokines. The levels of seminal plasma IL-17 and IL-12P70 in the oligoasthenospermia group and IL-1β and IL-12P70 in the azoospermia group were significantly higher than those in the normal routine semen parameters group (P ≤ 0.05), while the levels of IL-10 in the azoospermia group were significantly lower than that in the normal routine semen parameters group (P < 0.05).

Conclusion

There are certain correlations between seminal plasma cytokines and routine semen parameters and strong correlations between different seminal plasma cytokines, suggesting that the imbalance between seminal plasma cytokines may affect sperm quality. However, it still needs to be further confirmed by large samples and multi-center clinical studies and related basic researches.

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通过流式细胞术分析精浆中的 12 种细胞因子及其与精液常规参数的相关性。
目的:研究精浆中 12 种细胞因子的水平及其与精液常规参数的相关性:研究精浆中 12 种细胞因子的水平及其与精液常规指标的相关性:收集 134 名接受精液常规检查的患者的剩余精浆样本以检测细胞因子。采用计算机辅助精子分析(CASA)系统分析精子浓度、活动力精子(PR)百分比和活动力参数。根据精子浓度、活动率和活力的结果,134 名患者被分为精液常规参数正常组、少精子症组和无精子症组。采用流式细胞术检测精浆中 12 种细胞因子的水平,包括白细胞介素(IL)-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12P70、IL-17、干扰素(IFN)-α、IFN-γ 和肿瘤坏死因子(TNF)-α。两份精浆样本分别检测 10 次,以计算每种细胞因子的变异系数(CV)。使用标准品测定每种细胞因子的线性范围,并计算相关系数(r):流式细胞仪检测的 12 种细胞因子的 r2 均大于 0.99。2 份精浆样本的重现性显示,除样本 1 中 TNF-α 的 CV 值为 15.15%外,其他细胞因子的 CV 值均小于 15%。精液血浆中 IL-6 的水平与精液量呈负相关(P 结论:精液血浆中 IL-6 的水平与精液量呈负相关:精浆细胞因子与精液常规指标之间存在一定的相关性,不同精浆细胞因子之间存在较强的相关性,这表明精浆细胞因子之间的失衡可能会影响精子质量。不过,这还需要大样本、多中心临床研究和相关基础研究的进一步证实。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cytokine
Cytokine 医学-免疫学
CiteScore
7.60
自引率
2.60%
发文量
262
审稿时长
48 days
期刊介绍: The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.
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