5-Fluorouracil-Loaded PLGA Declined Expression of Pro-Inflammatory Genes IL-9, IL-17A, IL-23 and IFN- y; in the HT-29 Colon Cancer Cell Line.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2024-01-01 DOI:10.61186/rbmb.12.4.664
Basheer Kadhum Kharmeet, Mohammad Khalaj-Kondori, Mohammad Ali Hoseinpour Feizi, Jafar Hajavi
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Abstract

Background: Pro-inflammatory cytokines play critical roles in cancer pathobiology and have been considered potential targets for cancer management and therapy. Understanding the impact of cancer therapeutics such as 5-fluorouracil (5-FU) on their expression might shed light on development of novel combinational therapies. This study aimed to encapsulate 5-FU into PLGA and evaluate their effects on the expression of pro-inflammatory genes IL-9, IL-17-A, IL-23, and IFN-y; in the HT-29 cells.

Methods: PLGA-5-FU NPs were constructed and characterized by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). The cytotoxicity was evaluated by MTT test and, the IC50 was identified. HT-29 cells were treated with different concentrations of the PLGA-5-FU NPs for 48 hours and, gene expression levels were analyzed by qRT-PCR.

Results: DLS and AFM analysis revealed that the prepared PLGA-5-FU NPs were negatively charged spherical-shaped particles with a mean size of 215.9 ± 43.3 nm. PLGA-5-FU NPs impacted the viability of HT-29 cells in a dose- and time-dependent manner. The qRT-PCR results revealed a dose-dependent decrease in the expression of IL-9, IL-17A, IL-23 and IFN-y; genes, and their expressions were significantly different in both 10 and 20 µg/mL treated groups compared to the control. However, although the treatment of HT-29 cells with 20 µg/mL free 5-FU resulted in decreased expression of the studied genes, the differences were not statistically significant compared to the control group.

Conclusion: PLGA-5-FU NPs significantly suppressed expression of the IL-9, IL-17A, IL-23 and IFN-y; genes, and the encapsulation of 5-FU into PLGA improved considerably impact of the 5-FU on the HT-29 cells.

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5-Fluorouracil-Loaded PLGA 可降低 HT-29 结肠癌细胞系中促炎基因 IL-9、IL-17A、IL-23 和 IFN- y 的表达。
背景:促炎细胞因子在癌症病理生物学中起着至关重要的作用,被认为是癌症管理和治疗的潜在靶点。了解癌症治疗药物(如 5-氟尿嘧啶(5-FU))对其表达的影响可能有助于开发新型组合疗法。本研究旨在将 5-FU 封装到 PLGA 中,并评估其对 HT-29 细胞中促炎基因 IL-9、IL-17-A、IL-23 和 IFN-y 表达的影响:方法:构建了 PLGA-5-FU NPs,并通过动态光散射(DLS)和原子力显微镜(AFM)对其进行了表征。细胞毒性通过 MTT 试验进行评估,并确定了 IC50。用不同浓度的 PLGA-5-FU NPs 处理 HT-29 细胞 48 小时,并通过 qRT-PCR 分析基因表达水平:DLS和AFM分析表明,制备的PLGA-5-FU NPs为带负电荷的球形颗粒,平均大小为215.9 ± 43.3 nm。PLGA-5-FU NPs对HT-29细胞活力的影响呈剂量和时间依赖性。qRT-PCR结果显示,IL-9、IL-17A、IL-23和IFN-y基因的表达量呈剂量依赖性下降,与对照组相比,10和20 µg/mL处理组的表达量均有显著差异。然而,虽然用 20 µg/mL 游离 5-FU 处理 HT-29 细胞会导致所研究基因的表达量减少,但与对照组相比,差异无统计学意义:结论:PLGA-5-FU NPs能显著抑制IL-9、IL-17A、IL-23和IFN-y基因的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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