Advances in single-cell transcriptomics in animal research.

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Animal Science and Biotechnology Pub Date : 2024-08-02 DOI:10.1186/s40104-024-01063-y
Yunan Yan, Senlin Zhu, Minghui Jia, Xinyi Chen, Wenlingli Qi, Fengfei Gu, Teresa G Valencak, Jian-Xin Liu, Hui-Zeng Sun
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Abstract

Understanding biological mechanisms is fundamental for improving animal production and health to meet the growing demand for high-quality protein. As an emerging biotechnology, single-cell transcriptomics has been gradually applied in diverse aspects of animal research, offering an effective method to study the gene expression of high-throughput single cells of different tissues/organs in animals. In an unprecedented manner, researchers have identified cell types/subtypes and their marker genes, inferred cellular fate trajectories, and revealed cell‒cell interactions in animals using single-cell transcriptomics. In this paper, we introduce the development of single-cell technology and review the processes, advancements, and applications of single-cell transcriptomics in animal research. We summarize recent efforts using single-cell transcriptomics to obtain a more profound understanding of animal nutrition and health, reproductive performance, genetics, and disease models in different livestock species. Moreover, the practical experience accumulated based on a large number of cases is highlighted to provide a reference for determining key factors (e.g., sample size, cell clustering, and cell type annotation) in single-cell transcriptomics analysis. We also discuss the limitations and outlook of single-cell transcriptomics in the current stage. This paper describes the comprehensive progress of single-cell transcriptomics in animal research, offering novel insights and sustainable advancements in agricultural productivity and animal health.

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动物研究中单细胞转录组学的进展。
了解生物机制是提高动物生产和健康水平以满足对优质蛋白质日益增长的需求的基础。作为一种新兴的生物技术,单细胞转录组学已逐步应用于动物研究的各个方面,为研究动物不同组织/器官的高通量单细胞基因表达提供了一种有效的方法。研究人员利用单细胞转录组学以前所未有的方式鉴定了动物体内的细胞类型/亚型及其标记基因,推断了细胞命运轨迹,并揭示了细胞与细胞之间的相互作用。在本文中,我们将介绍单细胞技术的发展,并回顾单细胞转录组学在动物研究中的过程、进展和应用。我们总结了近年来利用单细胞转录组学更深入地了解不同家畜物种的动物营养与健康、繁殖性能、遗传学和疾病模型的工作。此外,我们还重点介绍了在大量案例基础上积累的实践经验,为确定单细胞转录组学分析的关键因素(如样本大小、细胞聚类和细胞类型注释)提供参考。我们还讨论了现阶段单细胞转录组学的局限性和前景。本文介绍了单细胞转录组学在动物研究中的全面进展,为农业生产力和动物健康提供了新的见解和可持续的进步。
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来源期刊
CiteScore
10.30
自引率
0.00%
发文量
822
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