The virulence plasmid associated with AHPND in shrimp appears to have originated from Vibrio owensii through a process of homologous recombination of parental plasmids and the transposable insertion of two large fragments

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-08-07 DOI:10.1016/j.jip.2024.108173
Yesheng Yan , Haojie Lu , Xiaosha Liang , Tianqi Xu , Shuling Yan , Yongxin Yu , Yongjie Wang
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Abstract

Acute hepatopancreatic necrosis disease (AHPND) is a highly contagious and lethal disease of shrimp caused by Vibrio strains carrying the virulence plasmid (pAHPND) containing the pirAB virulence genes. Through analysis of plasmid sequence similarity, clustering, and phylogeny, a horizontal transfer element similar to IS91 was discovered within the pAHPND plasmid. Additionally, two distinct clades of plasmids related to pAHPND (designated as pAHPND-r1 and pAHPND-r2) were identified, which may serve as potential parental plasmids for pAHPND. The available evidence, including the difference in G+C content between the plasmid and its host, codon usage preference, and plasmid recombination event prediction, suggests that the formation of the pAHPND plasmid in the Vibrio owensii strain was likely due to the synergistic effect of the recombinase RecA and the associated proteins RecBCD on the pAHPND-r1 and pAHPND-r2, resulting in the recombination and formation of the precursor plasmid for pAHPND (pre-pAHPND). The emergence of pAHPND was found to be a result of successive insertions of the horizontal transfer elements of pirAB-Tn903 and IS91-like segment, which led to the deletion of one third of the pre-pAHPND. This plasmid was then able to spread horizontally to other Vibrio strains, contributing to the epidemics of AHPND. These findings shed light on previously unknown mechanisms involved in the emergence of pAHPND and improve our understanding of the disease’s spread.

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与对虾 AHPND 相关的致病性质粒似乎是通过亲本质粒的同源重组和两个大片段的转座插入过程从 owensii 弧菌中产生的。
急性肝胰腺坏死病(AHPND)是由携带含有 pirAB 毒力基因的毒力质粒(pAHPND)的弧菌菌株引起的一种具有高度传染性和致命性的对虾疾病。通过分析质粒序列的相似性、聚类和系统发育,在 pAHPND 质粒中发现了与 IS91 相似的水平转移元件。此外,还发现了与 pAHPND 相关的两个不同的质粒支系(命名为 pAHPND-r1 和 pAHPND-r2),它们可能是 pAHPND 的潜在亲本质粒。现有的证据,包括质粒与其宿主之间 G+C 含量的差异、密码子使用偏好以及质粒重组事件预测等,都表明 pAHPND 的形成可能与基因重组有关、这些证据表明,pAHPND 质粒在奥文氏弧菌菌株中的形成可能是由于重组酶 RecA 和相关蛋白 RecBCD 对 pAHPND-r1 和 pAHPND-r2 的协同作用,导致重组并形成了 pAHPND 的前体质粒(pre-pAHPND)。研究发现,pAHPND 的出现是 pirAB-Tn903 水平转移元件和类 IS91 片段连续插入的结果,这导致前 pAHPND 的三分之一被删除。这种质粒随后能够水平传播到其他弧菌菌株,导致 AHPND 的流行。这些发现揭示了以前未知的 pAHPND 出现机制,提高了我们对该疾病传播的认识。
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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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