Assessment of Bilirubin Concentrations in the Bile of Patients with Obstructive Jaundice by Raman Spectroscopy

Abstract

Raman spectroscopy (RS) is widely used in biomedicine to detect various chemical compounds in substrates, as well as to determine their concentration. In this work, RS was used to detect bilirubin in the hepatic bile of patients with obstructive jaundice (OJ) of different etiologies obtained via a cholangiostomy drainage catheter. The spectral bands of bilirubin (1258–1264 and 1615–1620 cm^–1) were identified using the excitation wavelength of 785 nm. It was found that the assessment of bilirubin in bile allows to predict the recovery of excretory liver function and the dynamics of convalescence of patients with OJ after biliary decompression.