O. A. Averina, S. A. Kuznetsova, O. A. Permyakov, P. V. Sergiev
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引用次数: 0
Abstract
Modern genetic engineering technologies, such as base editing and prime editing (PE), have proven to provide the efficient and reliable genome editing tools that obviate the need for donor templates and double-strand breaks (DSBs) introduced in DNA. Relatively new, they quickly gained recognition for their accuracy, simplicity, and multiplexing capabilities. The review summarizes the new literature on the technologies and considers their architecture, methods to create editors, specificity, efficiency, and versatility. Advantages and disadvantages of the editors are discussed along with their prospective use in basic and applied research. The review may be useful for planning genome editing studies and analyzing their results to solve various problems of fundamental biology, biotechnology, medicine, and agriculture.
摘要 现代基因工程技术，如碱基编辑和质粒编辑（PE），已被证明是高效可靠的基因组编辑工具，无需供体模板和在 DNA 中引入双链断裂（DSB）。这些工具相对较新，但因其准确性、简便性和复用能力而迅速获得认可。这篇综述总结了有关这些技术的新文献，并考虑了它们的结构、创建编辑器的方法、特异性、效率和多功能性。文章讨论了编辑器的优缺点，以及它们在基础研究和应用研究中的应用前景。这篇综述可能有助于规划基因组编辑研究和分析其结果，以解决基础生物学、生物技术、医学和农业方面的各种问题。
期刊介绍：
Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.
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