Preparation of composite calcium phosphate cement scaffold loaded with Hedysarum polysaccharides and its efficacy in repairing bone defects

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of Materials Science: Materials in Medicine Pub Date : 2024-08-13 DOI:10.1007/s10856-024-06818-7
Lianggong Zhao, Bo Wang, Shilan Feng, Huifang Wu
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Abstract

It’s imperative to create a more ideal biological scaffold for bone defect repair. Calcium phosphate bone cements (CPC) could be used as a scaffold. Some ingredients and osteogenic factors could be added to improve its poor mechanical properties and biological activity. As a macromolecule extracted from traditional Chinese medicine, Hedysarum polysaccharides (HPS) would significantly promote the osteogenic activity of bone biomaterials. Zirconium oxide and starch were added to the solid phase and citric acid was added to the liquid phase to optimize CPC. HPS was loaded onto the scaffold as an osteogenic factor, and the prepared CPS + HPS was characterized. Further, the cytocompatibility of CPS + HPS was assessed according to activity, differentiation, and calcification in neonatal rat calvarial osteoblasts, and the biosafety of CPS + HPS was evaluated according to acute toxicity, pyrogen, sensitization, and hemolysis. The success of CPS + HPS in repairing bone defects was evaluated by using a rabbit femur implantation experiment. After optimization, CPS-20-CA-5 containing 10% starch and 5% citric acid displayed the highest mechanical strength of 28.96 ± 0.03 MPa. HPS-50 was demonstrated to exert the best osteogenic effect. The combination of CPS + HPS achieved HPS-loaded CPC. Material characterization, cytocompatibility, biosafety, and femoral implantation experiments indicated that CPS + HPS possessed better pressure resistance and improved osteogenic ability in bone defect repair.CPS + HPS demonstrated effective pressure resistance and superior osteogenic ability, which may be of great significance for bone defects and bone tissue engineering to promote bone regeneration and repair.

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负载 Hedysarum 多糖的复合磷酸钙水泥支架的制备及其在修复骨缺损中的功效。
当务之急是为骨缺损修复创造一种更理想的生物支架。磷酸钙骨水泥(CPC)可用作支架。可以添加一些成分和成骨因子来改善其不良的机械性能和生物活性。作为一种从传统中药中提取的大分子,海带多糖(HPS)可显著促进骨生物材料的成骨活性。在固相中加入氧化锆和淀粉,在液相中加入柠檬酸以优化 CPC。将 HPS 作为成骨因子添加到支架上,并对制备的 CPS + HPS 进行了表征。此外,还根据新生大鼠小腿成骨细胞的活性、分化和钙化情况评估了 CPS + HPS 的细胞相容性,并根据急性毒性、热原、致敏和溶血情况评估了 CPS + HPS 的生物安全性。通过兔股骨植入实验评估了 CPS + HPS 修复骨缺损的成功率。经过优化,含有 10%淀粉和 5%柠檬酸的 CPS-20-CA-5 显示出最高的机械强度(28.96 ± 0.03 兆帕)。HPS-50 的成骨效果最佳。CPS + HPS的组合实现了HPS负载CPC。材料表征、细胞相容性、生物安全性和股骨植入实验表明,CPS + HPS 在骨缺损修复中具有更好的抗压性和更强的成骨能力。
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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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