Sublethal Imidacloprid Administration to Honey Bee Workers is More Lethal to the Queen Larvae

IF 3.6 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Environmental Toxicology and Chemistry Pub Date : 2024-08-13 DOI:10.1002/etc.5965

Yun-Ru Chen, David T. W. Tzeng, Shih-Shun Lin, En-Cheng Yang

{"title":"Sublethal Imidacloprid Administration to Honey Bee Workers is More Lethal to the Queen Larvae","authors":"Yun-Ru Chen, David T. W. Tzeng, Shih-Shun Lin, En-Cheng Yang","doi":"10.1002/etc.5965","DOIUrl":null,"url":null,"abstract":"Imidacloprid and other neonicotinoid insecticides severely impact the performance and survival of honey bees and other pollinators. In the present study, we focused on the gene expression profile of newly emerged Apis mellifera queen bees after sublethal imidacloprid treatment during the larval stage. Royal jelly containing 1 ppb imidacloprid was provided to larvae for 3 consecutive days (2–4 days postemergence). Queen larvae treated with imidacloprid showed lower capping and emergence rates (35.5% and 24.22%, respectively) than did control larvae (61.68% and 52.95%, respectively), indicating a high failure rate of queen rearing associated with imidacloprid exposure during the larval stage. The molecular response to imidacloprid treatment was examined next. By comparing the gene expression profiles of imidacloprid-treated queen larvae and those of control queen larvae using DESeq2, we identified 215 differentially expressed genes, with 105 and 111 up- and downregulated genes, respectively. Gene Ontology results indicated that chitin binding– and calcium ion binding–related genes were upregulated, while phototransduction- and visual perception–related genes were downregulated. The high mortality rate and altered gene expression profiles suggest that treatment with even 1 ppb imidacloprid can severely impact queen bee survival. Environ Toxicol Chem 2024;43:2232–2242. © 2024 SETAC","PeriodicalId":11793,"journal":{"name":"Environmental Toxicology and Chemistry","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Toxicology and Chemistry","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/etc.5965","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Imidacloprid and other neonicotinoid insecticides severely impact the performance and survival of honey bees and other pollinators. In the present study, we focused on the gene expression profile of newly emerged Apis mellifera queen bees after sublethal imidacloprid treatment during the larval stage. Royal jelly containing 1 ppb imidacloprid was provided to larvae for 3 consecutive days (2–4 days postemergence). Queen larvae treated with imidacloprid showed lower capping and emergence rates (35.5% and 24.22%, respectively) than did control larvae (61.68% and 52.95%, respectively), indicating a high failure rate of queen rearing associated with imidacloprid exposure during the larval stage. The molecular response to imidacloprid treatment was examined next. By comparing the gene expression profiles of imidacloprid-treated queen larvae and those of control queen larvae using DESeq2, we identified 215 differentially expressed genes, with 105 and 111 up- and downregulated genes, respectively. Gene Ontology results indicated that chitin binding– and calcium ion binding–related genes were upregulated, while phototransduction- and visual perception–related genes were downregulated. The high mortality rate and altered gene expression profiles suggest that treatment with even 1 ppb imidacloprid can severely impact queen bee survival. Environ Toxicol Chem 2024;43:2232–2242. © 2024 SETAC

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

对蜜蜂工蜂施用亚致死浓度的吡虫啉对蜂王幼虫的杀伤力更大。

吡虫啉和其他新烟碱类杀虫剂严重影响蜜蜂和其他授粉昆虫的性能和生存。在本研究中，我们重点研究了幼虫期亚致死吡虫啉处理后新出蜂王的基因表达谱。连续 3 天（萌发后 2-4 天）向幼虫提供含有 1 ppb 吡虫啉的蜂王浆。经吡虫啉处理的王后幼虫的封盖率和出苗率（分别为 35.5% 和 24.22%）低于对照幼虫（分别为 61.68% 和 52.95%），这表明幼虫期接触吡虫啉会导致王后饲养的高失败率。接下来研究了吡虫啉处理的分子反应。通过使用 DESeq2 比较吡虫啉处理过的蜂后幼虫和对照蜂后幼虫的基因表达谱，我们发现了 215 个差异表达基因，其中上调基因和下调基因分别为 105 个和 111 个。基因本体的结果表明，与几丁质结合和钙离子结合相关的基因上调，而与光传导和视觉感知相关的基因下调。高死亡率和基因表达谱的改变表明，即使是1 ppb的吡虫啉也会严重影响蜂王的存活。环境毒物化学 2024;00:1-11。© 2024 SETAC.

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Environmental Toxicology and Chemistry 环境科学-毒理学

CiteScore

7.40

自引率

9.80%

发文量

265

审稿时长

3.4 months

期刊介绍： The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.

期刊最新文献

Correction. Spotlights are papers selected by editors published in peer-reviewed journals that may be more regionally specific or appearing in languages other than English Issue Information - Cover Editorial Board and Table of Contents Detection and Prediction of Toxic Aluminum Concentrations in High-Priority Salmon Rivers in Nova Scotia.