Integrated ceRNAs regulating relationship and bioinformatics analysis to study the molecular mechanisms of the inhibition of puerarin on bladder cancer cell.
Yu-Yang Ma, Wen Gao, Hao Wang, Hao Xu, Deng Pan, Jing-Kai Wang, Peng Xu, Hai-Luo Wang, Kun Pang
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引用次数: 0
Abstract
Based on previous experiments, we demonstrated puerarin inhibited the proliferation of BC T24 cells. To further explore the molecular mechanisms, whole transcriptome sequencing combined with bioinformatics analysis was performed. The results showed puerarin significantly inhibited T24 proliferation and pathway enrichment analysis of differentially expressed RNAs were mainly enriched in Cell cycle, PI3K/AKT, Ras family chromatin remodeling. lncRNAs and circRNAs may regulate miRNAs, thereby regulating the expression of ITGA1, PAK2 and UTRN. The predicted upstream transcription factor ERG and puerarin were well docked, which may be one of the underlying mechanisms by which puerarin inhibiting BC cells.
基于之前的实验，我们证实葛根素能抑制 BC T24 细胞的增殖。为了进一步探讨其分子机制，我们进行了全转录组测序和生物信息学分析。结果表明，葛根素能明显抑制T24细胞的增殖，其差异表达RNA的通路富集分析主要富集于细胞周期、PI3K/AKT、Ras家族染色质重塑。预测的上游转录因子ERG与葛根素有很好的对接，这可能是葛根素抑制BC细胞的潜在机制之一。
期刊介绍：
The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures.
All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.
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