Pediatric nephrotic syndrome: The interplay of oxidative stress and inflammation.

IF 2 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Medical Biochemistry Pub Date : 2024-06-15 DOI:10.5937/jomb0-46526
Simachew Yonas Mulat, Marija Mihajlović, Tamara Antonić, Gordana Miloševski-Lomić, Amira Peco-Antić, Dragana Jovanović, Dušan Paripović, Aleksandra Stefanović
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Abstract

Background: The pathophysiological mechanisms crucial in the development of nephrotic syndrome (NS) in the pediatric population are still not fully understood. This study aimed to investigate the relationship between hypertension, oxidative stress, and inflammation in pediatric patients during the acute phase of the disease.

Methods: The study included 33 children, aged 2 to 9 years, with nephrotic syndrome. Blood samples were collected during the acute phase and remission. Parameters of oxidative status were determined, including total oxidative status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB), sulfhydryl groups (-SH), paraoxonase 1 (PON1), and total antioxidant status (TAS) in serum, measured spectrophotometrically. Inflam - matory parameters such as pentraxin 3 (PTX3), leptin, program med cell death ligand 1 (PD-L1), and E-cadherin were determined using enzyme-linked immunosorbent assay (ELISA).

Results: Patients with nephrotic syndrome and hypertension had significantly higher levels of advanced oxidation protein products and total antioxidant status (p=0.029 and p=0.003, respectively). During the acute phase of the disease, lower activity of sulfhydryl groups and paraoxonase 1 was observed compared to remission (p<0.001, for both). Pentraxin 3 levels were higher, while leptin levels were lower during the acute phase (p<0.001, for both). Pentraxin 3 correlated with advanced oxidation protein products and total antioxidant status during the acute phase but not in remission (rs=0.42, p=0.027 and rs=0.43, p=0.025, respectively). A negative correlation between Advanced oxidation protein products and leptin was observed during the acute phase, which disappeared in remission (rs=-0.42, p=0.028).

Conclusions: Results of this study show that hypertension influences oxidative stress markers, and decreased antioxidant capacity may contribute to nephrotic syndrome development. Pentraxin 3 appears as a potential disease activity marker, indicating a dynamic connection between inflammation and oxidative stress. Leptin may also play a role in oxidative stress in nephrotic syndrome.

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小儿肾病综合征:氧化应激和炎症的相互作用
背景:小儿肾病综合征(NS)发病的关键病理生理机制尚未完全明了。本研究旨在探讨小儿肾病综合征急性期高血压、氧化应激和炎症之间的关系:研究对象包括33名肾病综合征患儿,年龄在2至9岁之间。在急性期和缓解期收集血液样本。通过分光光度法测定了血清中的氧化状态参数,包括总氧化状态(TOS)、高级氧化蛋白产物(AOPP)、原氧化剂-抗氧化剂平衡(PAB)、巯基(-SH)、副氧自由基酶 1(PON1)和总抗氧化剂状态(TAS)。使用酶联免疫吸附试验(ELISA)测定五肽 3(PTX3)、瘦素、程序介导细胞死亡配体 1(PD-L1)和 E-粘连蛋白等炎症参数:结果:肾病综合征和高血压患者的高级氧化蛋白产物和总抗氧化状态水平明显更高(分别为P=0.029和P=0.003)。在疾病急性期,与缓解期相比,巯基和副氧合酶 1 的活性较低(p结论:这项研究的结果表明,高血压会影响氧化应激标记物,而抗氧化能力下降可能会导致肾病综合征的发生。五胜肽 3 似乎是一种潜在的疾病活动标志物,表明炎症和氧化应激之间存在动态联系。瘦素也可能在肾病综合征的氧化应激中发挥作用。
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来源期刊
Journal of Medical Biochemistry
Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
12.00%
发文量
60
审稿时长
>12 weeks
期刊介绍: The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.
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