Morphology, complete mitochondrial genome, and molecular phylogeny of Rhabdias macrocephalum n. sp. (Nematoda: Rhabdiasidae) from Diploderma splendidum (Reptilia: Agamidae).

IF 2.3 2区医学 Q2 PARASITOLOGY Parasite Pub Date : 2024-01-01 Epub Date: 2024-08-14 DOI:10.1051/parasite/2024046

Jia-Lu Zeng, Hui-Xia Chen, Hong-Ru Xu, Liang Li

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Abstract

Species of the genus Rhabdias Stiles & Hassall, 1905 are common parasitic nematodes occurring in the lungs of amphibians and reptiles worldwide. In the present study, Rhabdias macrocephalum n. sp. is described using integrated morphological methods (light and scanning electron microscopy) and molecular approaches (sequencing of the nuclear 28S and ITS regions, and mitochondrial cox1, cox2, and 12S genes) based on specimens collected from the green striped tree dragon Diploderma splendidum (Barbour & Dunn) (Reptilia: Agamidae) in China. The complete mitochondrial genome of R. macrocephalum n. sp. was sequenced and annotated: it is 14,819 bp in length, including 12 protein coding genes (missing atp8), 22 tRNA genes, 2 rRNA genes and three non-coding regions. The gene arrangement of R. macrocephalum n. sp. is different from all of the currently available mitogenomes of nematodes and represents a novel type of mitochondrial gene arrangement reported in Nematoda. Molecular phylogenetic results based on the ITS + 28S data support the monophyly of Entomelas, Pneumonema, Serpentirhabdias, and Rhabdias, and showed R. macrocephalum n. sp. forming a most basal lineage in Rhabdias.

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来自Diploderma splendidum (Reptilia: Agamidae) 的Rhabdias macrocephalum n. sp.（线虫纲：Rhabdiasidae）的形态学、完整线粒体基因组和分子系统发育。

Rhabdias Stiles & Hassall, 1905 属的物种是寄生于全球两栖类和爬行类动物肺部的常见线虫。本研究基于从中国绿纹树龙（Diploderma splendidum (Barbour & Dunn) (Reptilia: Agamidae)）采集的标本，采用综合形态学方法（光学显微镜和扫描电子显微镜）和分子方法（核28S和ITS区测序，线粒体cox1、cox2和12S基因测序）对大头瘤鼠（Rhabdias macrocephalum n. sp.）进行了描述。大头龙的线粒体基因组全长 14,819 bp，包括 12 个蛋白编码基因（缺少 atp8）、22 个 tRNA 基因、2 个 rRNA 基因和 3 个非编码区。R. macrocephalum n. sp.的基因排列不同于目前所有线虫的有丝分裂基因组，代表了线虫纲线粒体基因排列的一种新类型。基于 ITS + 28S 数据的分子系统学结果支持 Entomelas、Pneumonema、Serpentirhabdias 和 Rhabdias 的单系性，并显示 R. macrocephalum n. sp.

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.