Inhibition of Norovirus GII.4 binding to HBGAs by Sargassum fusiforme polysaccharide.

IF 3.8 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioscience Reports Pub Date : 2024-09-25 DOI:10.1042/BSR20240092

Yiqiang Sun, Meina Liang, Mingjiang Wu, Laijin Su

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Abstract

Norovirus (NoV) is the main pathogen that causes acute gastroenteritis and brings a heavy socio-economic burden worldwide. In this study, five polysaccharide fractions, labeled pSFP-1-5, were isolated and purified from Sargassum fusiforme (S. fusiforme). In vitro experiments demonstrated that pSFP-5 significantly prevented the binding of type A, B and H histo-blood group antigens (HBGAs) to NoV GII.4 virus-like particles (NoV GII.4 VLPs). In addition, in vivo experiments revealed that pSFP-5 was effective in reducing the accumulation of NoV in oysters, indicating that pSFP-5 could reduce the risk of NoV infection from oyster consumption. The results of transmission electron microscopy showed that the appearance of NoV GII.4 VLPs changed after pSFP-5 treatment, indicating that pSFP-5 may achieve antiviral ability by altering the morphological structure of the viral particles so that they could not bind to HBGAs. The results of the present study indicate that pSFP-5 may be an effective anti-NoV substance and can be used as a potential anti-NoV drug component.

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马尾藻多糖抑制诺罗病毒 GII.4 与 HBGAs 的结合。

诺如病毒（NoV）是引起急性肠胃炎的主要病原体，给全世界带来了沉重的社会经济负担。本研究从马尾藻（Sargassum fusiforme，S. fusiforme）中分离纯化出五种多糖组分，标记为 pSFP-1-5。体外实验表明，pSFP-5 能显著阻止 A、B 和 H 型组织血型抗原（HBGAs）与 NoV GII.4 病毒样颗粒（NoV GII.4 VLPs）的结合。此外，体内实验显示，pSFP-5 能有效减少 NoV 在牡蛎中的积累，这表明 pSFP-5 能降低食用牡蛎感染 NoV 的风险。透射电子显微镜结果显示，经过 pSFP-5 处理后，NoV GII.4 VLPs 的外观发生了变化，这表明 pSFP-5 可能通过改变病毒颗粒的形态结构，使其无法与 HBGAs 结合，从而达到抗病毒的目的。本研究结果表明，pSFP-5 可能是一种有效的抗野病毒物质，可用作潜在的抗野病毒药物成分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience Reports 生物-细胞生物学

CiteScore

8.50

自引率

0.00%

发文量

380

审稿时长

6-12 weeks

期刊介绍： Bioscience Reports provides a home for sound scientific research in all areas of cell biology and molecular life sciences. Since 2012, Bioscience Reports has been fully Open Access and publishes all papers under the liberal CC BY licence, giving the life science community quality research to share and discuss.Content before 2012 is subscription-only, and is accessible via archive purchase. Articles are assessed on soundness, providing a home for valid findings and data. We welcome papers that span disciplines (e.g. chemistry, medicine), including papers describing: -new methodologies -tools and reagents to probe biological questions -mechanistic details -disease mechanisms -metabolic processes and their regulation -structure and function -bioenergetics