Isolation and molecular characterization of fowl adenovirus from inclusion body hepatitis cases in Western India during 2019-2021.

IF 0.8 4区 农林科学 Q3 VETERINARY SCIENCES Iranian journal of veterinary research Pub Date : 2024-01-01 DOI:10.22099/IJVR.2024.47035.6773
V G Chavan, S P Awandkar, R C Kulkarni, S G Chavhan, R D Suryawanshi, R K Jadhav, M B Kulkarni, A A Agnihotri
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Abstract

Background: Inclusion body hepatitis (IBH) resulted in a substantial economic loss in Western India during 2019 to 2021.

Aims: The study aimed to characterize fowl adenovirus (FAdV) from field outbreaks.

Methods: The study was conducted on 290 liver samples from 66 poultry flocks. The samples were subjected to histopathology and molecular detection, followed by phylogenetic typing of the partial hexon gene of the virus.

Results: Spiking mortality (14%) was recorded from day 21 to day 35 with peak mortality at the 28th day of age. The necropsy showed a pale and enlarged liver with hemorrhagic and yellowish necrotic foci, accumulation of straw-colored transudate in the pericardial sac which resulted in a flabby appearance of the heart, heart enlargement, and hemorrhages on the spleen, enlarged and congested kidneys. The virus inoculation resulted in stunting and poor feathering with hepatomegaly, hemorrhages and yellowish necrotic foci on the liver as well as greenish discoloration, and kidney swelling in SPF embryonated chicken eggs. Out of 29, 16 liver samples yielded 1219 bp amplicons specific to hexon gene fragments. The sequence and phylogenetic analysis identified 14 isolates as FAdV species E serotype 11 and two as species D serotype 8b. Conclusion: The results indicated that FAdV-8b and FAdV-11 strains are involved in disease outbreaks in western India.

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从 2019-2021 年印度西部包涵体肝炎病例中分离鸡腺病毒并确定其分子特征。
背景:2019年至2021年期间,印度西部的包涵体肝炎(IBH)造成了巨大的经济损失。研究目的:本研究旨在分析田间疫情中鸡腺病毒(FAdV)的特征:研究对来自 66 个禽群的 290 份肝脏样本进行了检测。对样本进行组织病理学和分子检测,然后对病毒的部分 hexon 基因进行系统发育分型:从第 21 天到第 35 天,死亡率呈上升趋势(14%),第 28 日龄时死亡率最高。尸体解剖显示肝脏苍白、肿大,有出血和淡黄色坏死灶,心包囊积聚稻草色渗出物,导致心脏外观松软、心脏肿大、脾脏出血、肾脏肿大和充血。病毒接种导致 SPF 胚胎蛋鸡发育不良、羽毛稀疏、肝脏肿大、出血、肝脏上有淡黄色坏死灶、变绿以及肾脏肿大。在 29 个样本中,16 个肝脏样本产生了 1219 bp 的特异性 hexon 基因片段扩增子。序列和系统进化分析确定 14 个分离株为 FAdV E 血清型 11 种,2 个为 D 血清型 8b 种。结论结果表明,FAdV-8b 和 FAdV-11 株系参与了印度西部的疾病暴发。
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来源期刊
CiteScore
1.70
自引率
8.30%
发文量
10
期刊介绍: The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
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