A smartphone-based immunochromatographic strip platform for on-site quantitative detection of antigenic targets†

IF 6.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Lab on a Chip Pub Date : 2024-08-19 DOI:10.1039/D4LC00484A

Enhui Zhang, Qing Zeng, Yanwen Xu, Jinhui Lu, Chengcheng Li, Ke Xiao, Xiaozhou Li, Jinfeng Li, Tingting Li, Chengyao Li and Ling Zhang

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Abstract

To report the testing signal of an immunochromatographic assay for on-site quantitative detection, a portable and user-friendly smartphone-based biosensing platform is developed in this study. This innovative system is composed of an ambient light sensor inherent smartphone reader and a 3D-printed handhold device, a quantitative tool capable of directly interpreting carbon nanoparticle (CNP)-conjugated immunochromatographic strips. To showcase the platform capability, the smartphone-based immunochromatography system (SPICS) reader and device were successfully used in CNP strips for rapid detection of the early pregnancy marker human chorionic gonadotropin in female urine (HCG; limit of detection [LOD]: 0.30 mIU mL⁻¹), prostate-specific antigen in patient blood (PSA; LOD: 0.28 ng mL⁻¹) and ampicillin residue in animal milk (AMP; LOD: 0.23 ng mL⁻¹). The results were fully correlated with conventional commercial instruments (R² = 0.99). The SPICS platform exhibits significant advantages, including portability, cost-effectiveness, easy operation, and rapid and quantitative detection, making it a valuable on-site diagnosis tool for use in home and community healthcare facilities.

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基于智能手机的免疫层析条带平台，用于现场定量检测抗原靶标

为了报告用于现场定量检测的免疫层析检测信号，本研究开发了一种基于智能手机的便携式、用户友好型生物传感平台。这一创新系统由环境光传感器、固有的智能手机阅读器和 3D 打印手持装置组成，是一种能够直接解读碳纳米粒子（CNPs）共轭免疫层析条带的定量工具。为了展示该平台的能力，我们成功地将基于智能手机的免疫层析系统（SPICS）读取器和装置用于 CNPs-条带，以快速检测女性尿液中的早孕标志物人类绒毛膜促性腺激素（HCG；检测限 [LOD]：0.30 mIU mL-1）、患者血液中的前列腺特异性抗原（PSA；检测限：0.28 ng mL-1）和动物乳汁中的氨苄西林残留量（AMP；检测限：0.23 ng mL-1）。结果与传统商业仪器完全相关（R2 = 0.99）。SPICS 平台具有便携、经济、易操作、快速和定量检测等显著优势，是家庭和社区医疗机构的重要现场诊断工具。

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来源期刊

Lab on a Chip 工程技术-化学综合

CiteScore

11.10

自引率

8.20%

发文量

434

审稿时长

2.6 months

期刊介绍： Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.