Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-08-22 DOI:10.1016/j.jgar.2024.08.003
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Abstract

Colistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene mcr-1 has become a public health threat. In this study, we identified mcr-1-harboring Leclercia adecarboxylata strain (WWCOL-134) isolated from wastewater in Seoul. The strain had a colistin MIC value of 2 µg/ml and was resistant to cefotaxime, gentamicin, tetracycline, trimethoprim and sulfamethoxazole. The mcr-1 gene, along with an array of resistance genes, was located on a 236-kb plasmid (pCOL134-1), which contained the typical IncHI2 backbone of reported mcr-1-carrying plasmids, and was transferred to an Escherichia coli strain by conjugation. To the best of our knowledge, this is the first study to report the emergence of mcr-1-harboring Leclercia sp. isolate. Our findings demonstrate the ongoing spread of colistin resistance among Enterobacterales species, emphasizing the need for surveillance of antimicrobial resistance in wastewater environments.

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从首尔废水中分离出的一种 adecarboxylata Leclercia 菌株中出现了可移动的可乐定抗性基因 mcr-1。
可乐定被认为是治疗耐多药细菌感染的最后手段。然而,质粒携带的耐秋水仙碱基因 mcr-1 的传播已成为一种公共卫生威胁。在本研究中,我们发现了从首尔废水中分离出的携带 mcr-1 的 Leclercia adecarboxylata 菌株(WWCOL-134)。该菌株对可乐菌素的 MIC 值为 2 µg/ml,对头孢他啶、庆大霉素、四环素、三甲双胍和磺胺甲噁唑有抗药性。mcr-1 基因和一系列抗性基因位于一个 236 kb 的质粒(pCOL134-1)上,该质粒含有已报道的携带 mcr-1 的质粒的典型 IncHI2 主干,并通过共轭转入大肠杆菌菌株。据我们所知,这是首次报道出现携带 mcr-1 的大肠杆菌分离物的研究。我们的研究结果表明,肠杆菌科细菌对可乐定的耐药性正在不断扩散,这强调了对废水环境中抗菌素耐药性进行监控的必要性。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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