Off–On fluorescent benzothiazole-fused coumarin for sensitive detection of nitroreductases and hydrogen sulfide

IF 1.7 4区 化学 Bulletin of the Korean Chemical Society Pub Date : 2024-08-16 DOI:10.1002/bkcs.12889
Song Yi Yoo, Na Yoon Kim, Min Hee Lee
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Abstract

Hydrogen sulfide (H2S) and nitroreductases (NTR) are key biological reductants, with H2S acting as a key signaling molecule and NTR reducing nitro groups to amines in cellular metabolism. Visualizing these reducing components, often overexpressed in cancer cells or bacterial environments, is crucial for metabolic understanding. Existing fluorescent probes detect these individually, but simultaneous detection methods are unexplored. We designed probe 1, which shows high fluorescence only when both NTR and H2S are overexpressed. The probe transformed into benzothiazole-fused coumarin by NTR, exhibiting fluorescence, and shows a stronger signal in the presence of both NTR and H2S, making it a potential tool for detecting their simultaneous overexpression.

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用于灵敏检测硝基还原酶和硫化氢的离体荧光苯并噻唑融合香豆素
硫化氢(H2S)和硝基还原酶(NTR)是关键的生物还原剂,其中 H2S 是关键的信号分子,而 NTR 则在细胞代谢过程中将硝基还原成胺。这些还原成分通常在癌细胞或细菌环境中过度表达,可视化它们对于了解新陈代谢至关重要。现有的荧光探针可以单独检测这些成分,但同时检测的方法还没有被探索出来。我们设计了探针 1,它只有在 NTR 和 H2S 同时过表达时才会显示高荧光。该探针通过 NTR 转化为苯并噻唑融合香豆素,显示出荧光,并在 NTR 和 H2S 同时存在时显示出更强的信号,使其成为检测 NTR 和 H2S 同时过表达的潜在工具。
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来源期刊
Bulletin of the Korean Chemical Society
Bulletin of the Korean Chemical Society Chemistry-General Chemistry
自引率
23.50%
发文量
182
期刊介绍: The Bulletin of the Korean Chemical Society is an official research journal of the Korean Chemical Society. It was founded in 1980 and reaches out to the chemical community worldwide. It is strictly peer-reviewed and welcomes Accounts, Communications, Articles, and Notes written in English. The scope of the journal covers all major areas of chemistry: analytical chemistry, electrochemistry, industrial chemistry, inorganic chemistry, life-science chemistry, macromolecular chemistry, organic synthesis, non-synthetic organic chemistry, physical chemistry, and materials chemistry.
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