The lipoteichoic acid of Lactobacillus plantarum effect on lymphocyte, VEGF-A and TGF-β expression in male rat dental pulp

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Cytokine Pub Date : 2024-08-24 DOI:10.1016/j.cyto.2024.156741

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Abstract

Objective

Lipoteichoic acid from Lactobacillus plantarum (L. plantarum) is a significant virulence factor that exacerbates pulp inflammation. Lipoteichoic acid plays a role in modulating the inflammatory to proliferative phase transition is crucial in determining the outcome of pulp healing or necrosis. This study explores the role of L. plantarum on lymphocytes and the expression of transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor A (VEGF-A) in a male rat model of acute dental pulp injury.

Design

The acute dental pulp model was created in the upper molar of Rattus novergicus using a round bur. Then, the dental pulp was exposed to 10 µg/ml of the lipoteichoic acid of L. plantarum and filled with a temporary filling. In the next 24, 48, and 72 h, each animal was decapitated, and the expression of TGF-β1 and VEGF-A in dental pulp was analyzed using indirect immunohistochemistry, while the lymphocytes analyzed using hematoxyline-eosin staining.

Result

Lipoteichoic acid of L. plantarum induced acute dental pulp by increasing the lymphocyte number after 48 and 72 h of exposure (p < 0.05). While, inhibiting TGF-β1 expression after 48 and 72 h of exposure (p < 0.05), and VEGF-A was inhibiting after 72 h of exposure (p < 0.05).

Conclusion

Exposure to lipoteichoic acid from L. plantarum significantly accelerates the inflammatory response in the dental pulp. However, this accelerated inflammation disrupts the proliferative phase, potentially leading to more extensive damage to the dental pulp.

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植物乳杆菌的脂联素对雄性大鼠牙髓淋巴细胞、血管内皮生长因子-A 和 TGF-β 表达的影响

目的：植物乳杆菌（L. plantarum）产生的脂联素是一种重要的毒力因子，会加剧牙髓炎症。脂联素酸在炎症向增殖阶段的转变过程中起着调节作用，对决定牙髓愈合或坏死的结果至关重要。本研究探讨了植物乳杆菌在急性牙髓损伤雄性大鼠模型中对淋巴细胞以及转化生长因子β1（TGF-β1）和血管内皮生长因子A（VEGF-A）表达的作用：设计：用圆钻在大鼠上臼齿中制作急性牙髓模型。然后，将牙髓暴露于 10 µg/ml 的植物酵母菌脂联素酸中，并用临时填充物填充。在接下来的24、48和72小时内，将每只动物断头，用间接免疫组化法分析牙髓中TGF-β1和VEGF-A的表达，用苏木精-伊红染色法分析淋巴细胞的表达：结果：植物酵母的脂联素诱导急性牙髓炎，暴露 48 和 72 小时后淋巴细胞数量增加（p 结论：植物酵母的脂联素诱导急性牙髓炎，暴露 48 和 72 小时后淋巴细胞数量增加（p 结论：植物酵母的脂联素诱导急性牙髓炎：接触植物乳杆菌的脂联乳酸会明显加速牙髓的炎症反应。然而，这种加速的炎症反应会破坏增殖阶段，从而可能导致牙髓受到更广泛的损害。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.