Lipopolysaccharide Derived from Pantoea agglomerans Directly Promotes the Migration of Human Keratinocytes.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL In vivo Pub Date : 2024-09-01 DOI:10.21873/invivo.13680

Hiroyuki Inagawa, Takashi Nishizawa, Chie Kohchi, Gen-Ichiro Soma

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Abstract

Background/aim: Because the skin is exposed to the external environment, it is important that wound healing processes proceed and terminate rapidly to minimize the risk of infection. A previous case report described the promotion of wound healing by transdermal administration of lipopolysaccharide derived from Pantoea agglomerans (LPSp). However, whether the wound healing-promoting effect of LPSp was due to direct activity on skin cells or indirect effects involving macrophages remained unclear. Therefore, this study investigated the wound healing-promoting effect of LPSp, particularly the promotion of keratinocyte migration.

Materials and methods: The migration of HaCaT human keratinocytes over time with and without LPSp was assayed using a cell migration assay kit. Migration was also analyzed using HaCaT cells treated with LPSp and an antibody against Toll-like receptor (TLR) 4, a receptor for LPS.

Results: Addition of LPSp significantly enhanced cell migration compared to no LPSp addition. Migration was inhibited by the addition of anti-TLR4 antibody.

Conclusion: LPSp acts directly on epidermal cells to promote migration and may be one mechanism by which LPSp promotes wound healing.

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盘尾丝菌产生的脂多糖直接促进人类角质形成细胞的迁移

背景/目的：由于皮肤暴露在外部环境中，因此伤口愈合过程必须迅速进行和结束，以最大限度地降低感染风险。之前的一份病例报告描述了通过透皮给药从盘尾丝菌（Pantoea agglomerans）中提取的脂多糖（LPSp）促进伤口愈合的情况。然而，LPSp 促进伤口愈合的作用是直接作用于皮肤细胞，还是间接作用于巨噬细胞，目前仍不清楚。因此，本研究探讨了 LPSp 的伤口愈合促进作用，尤其是促进角质细胞迁移的作用：使用细胞迁移检测试剂盒检测了有 LPSp 和无 LPSp 时 HaCaT 人角质形成细胞的迁移情况。此外，还使用经 LPSp 和 LPS 受体 Toll 样受体 (TLR) 4 抗体处理的 HaCaT 细胞对迁移进行了分析：结果：与不添加 LPSp 相比，添加 LPSp 能明显增强细胞迁移。结论：LPSp 可直接作用于表皮：结论：LPSp 直接作用于表皮细胞以促进迁移，这可能是 LPSp 促进伤口愈合的机制之一。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.