Seroprevalence and molecular detection of Brucella infection in livestock in the United Arab Emirates.

IF 2.8 Q1 VETERINARY SCIENCES International Journal of Veterinary Science and Medicine Pub Date : 2024-08-23 eCollection Date: 2024-01-01 DOI:10.1080/23144599.2024.2384015
Gobena Ameni, Aboma Zewude, Berecha Bayissa, Ibrahim Abdalla Alfaki, Abdallah A Albizreh, Naeema Alhosani, Khaja Mohteshamuddin, Berhanu Adenew Degefa, Mohamed Elfatih Hamad, Meera Saeed Alkalbani, Mohamed Moustafa Abdelhalim, Assem Sobhi Abdelazim, Rafeek Aroul Koliyan, Kaltham Kayaf, Mervat Mari Al Nuaimat, Robert Barigye, Arve Lee Willingham, Markos Tibbo, Bedaso Mammo Edo, Teshale Sori, Yassir Mohammed Eltahir
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Abstract

Small ruminants and camels are important livestock species in the United Arab Emirates (UAE), although Brucella infection can limit their productivity. This study aimed to investigate the seroprevalence of Brucella infection and its associated risk factors in small ruminants and camels in the Emirate of Abu Dhabi. Additionally, seropositive animals were tested for the DNA of Brucella. Multispecies competitive enzyme-linked immunosorbent assay (c-ELISA) and multispecies indirect (i-ELISA) were used to test 3,086 animals from 2022 to 2023. Brucella cell surface 31 kDa protein (bcsp31) gene-based real-time polymerase chain reaction (q-PCR) was used to detect Brucella DNA. Multivariate logistic regression was used to assess the association between seroprevalence and potential risk factors. The overall seroprevalences of Brucella infection were 1.7% (95% confidence interval [CI], 1.2%-2.2%) and 5.8% (95% CI, 5.0%-6.7%) based on serial and parallel testing, respectively. The DNA of Brucella was detected in 13 of the 51 seropositive animals. The overall seroprevalence of Brucella infection was associated with the region, type of animal holding, species, and age of the animals. In conclusion, this study documented Brucella infection in small ruminants and camels in the Emirate of Abu Dhabi, warranting necessary intervention strategies to eliminate Brucella infections in livestock populations.

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阿拉伯联合酋长国牲畜布鲁氏菌感染的血清流行率和分子检测。
小反刍动物和骆驼是阿拉伯联合酋长国(UAE)的重要牲畜物种,但布鲁氏杆菌感染会限制它们的生产力。本研究旨在调查阿布扎比酋长国小型反刍动物和骆驼的布鲁氏菌感染血清阳性率及其相关风险因素。此外,还对血清阳性动物进行了布鲁氏菌 DNA 检测。从2022年到2023年,采用多菌种竞争性酶联免疫吸附试验(c-ELISA)和多菌种间接免疫吸附试验(i-ELISA)对3086只动物进行了检测。布鲁氏菌细胞表面 31 kDa 蛋白 (bcsp31) 基因实时聚合酶链反应 (q-PCR) 用于检测布鲁氏菌 DNA。多变量逻辑回归用于评估血清流行率与潜在风险因素之间的关联。根据序列检测和平行检测,布鲁氏菌感染的总体血清流行率分别为 1.7%(95% 置信区间 [CI],1.2%-2.2%)和 5.8%(95% CI,5.0%-6.7%)。在51只血清反应呈阳性的动物中,有13只检测到布鲁氏菌的DNA。布鲁氏菌感染的总体血清流行率与地区、动物饲养类型、物种和动物年龄有关。总之,本研究记录了阿布扎比酋长国小型反刍动物和骆驼的布鲁氏菌感染情况,因此需要采取必要的干预策略来消除牲畜中的布鲁氏菌感染。
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来源期刊
CiteScore
4.80
自引率
0.00%
发文量
12
审稿时长
24 weeks
期刊最新文献
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