Labeled sandwich-type electrochemical immunosensor based on Ti3C2Tx/AuNP and Ti3C2Tx/HKUST-1/TB composites for early liver cancer detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2024-08-27 DOI:10.1007/s00604-024-06618-4

Haotian Wu, Gang Zhang, Xiaozhan Yang

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Abstract

A novel sandwich-type electrochemical immunosensor for the detection of the liver cancer marker alpha-fetoprotein (AFP) in human serum is proposed. The two-dimensional MXene material Ti₃C₂T_x was first prepared using etching and ultrasonic stripping, and then Ti₃C₂T_x was used to reduce chloroauric acid to form Ti₃C₂T_x/AuNP composites which were modified on the surface of the glassy carbon electrodes to form probe-type sensors. The Ti₃C₂T_x/AuNPs provide a large number of binding sites for the AFP capture antibody (Ab1) and increase the electrochemical reaction active site. The Ti₃C₂T_x/copper metal–organic frameworks HKUST-1 composite was also prepared by solvothermal method and combined with toluidine blue (TB) and AFP detection antibody (Ab2) to form a labeled sandwich-type electrochemical immunosensor. The sensor achieved trace detection of AFP from 0.1 to 100 ng/mL with a detection limit of 0.073 pg/mL and possesses good selectivity, stability, and reproducibility. The sensor performs well in clinical samples and has good potential for clinical applications.

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基于 Ti3C2Tx/AuNP 和 Ti3C2Tx/HKUST-1/TB 复合物的标记夹心型电化学免疫传感器用于早期肝癌检测。

本文提出了一种新型夹层式电化学免疫传感器，用于检测人血清中的肝癌标志物甲胎蛋白（AFP）。首先用刻蚀和超声剥离法制备了二维 MXene 材料 Ti3C2Tx，然后用 Ti3C2Tx 还原氯金酸形成 Ti3C2Tx/AuNP 复合材料，并将其修饰在玻璃碳电极表面形成探针型传感器。Ti3C2Tx/AuNPs 为 AFP 捕获抗体（Ab1）提供了大量结合位点，增加了电化学反应活性位点。此外，还采用溶热法制备了 Ti3C2Tx/铜金属有机框架 HKUST-1 复合材料，并将其与甲苯胺蓝（TB）和 AFP 检测抗体（Ab2）结合，形成了标记夹心型电化学免疫传感器。该传感器可痕量检测 0.1 至 100 ng/mL 的甲胎蛋白，检测限为 0.073 pg/mL，具有良好的选择性、稳定性和重现性。该传感器在临床样本中表现良好，具有很好的临床应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.