Effect of the estrous cycle on zinc transporter expression in bovine cumulus-oocyte complexes and oviduct epithelial cells.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI:10.1007/s11259-024-10523-9

A M Pascua, G Barbisan, N Nikoloff, A C Carranza-Martín, M C Fabra, J P Anchordoquy, M Balbi, C Furnus, J M Anchordoquy

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Abstract

During the luteal and follicular phases of the estrous cycle, cumulus-oocyte complexes (COC) and oviduct epithelial cells (OEC) undergo notable physiological and morphological changes. Maintaining proper zinc (Zn) homeostasis is crucial in both somatic and germinal mammalian cells. This study aimed to assess the impact of the estrous phase (luteal or follicular) on Zn transporter expression in bovine COC and OEC (BOEC). The expression of Zn transporters Slc39a6 (ZIP6), Slc39a8 (ZIP8), Slc39a14 (ZIP14), Slc30a3 (ZnT3), Slc30a7 (ZnT7), and Slc30a9 (ZnT9) was analyzed in COC and BOEC from cows during the luteal or follicular phases. Gene expression of ZIP6, ZIP14, and ZnT9 was quantified in COC and BOEC. The gene expression in the remaining transporters could not be quantified due to low mRNA levels (ZIP8 and ZnT3 in COC and BOEC; ZnT7 in BOEC) or absence of expression (ZnT7 in COC). In COC, the relative expression (RE) of all three transporters was higher in the luteal phase compared to the follicular phase (P ≤ 0.05). In BOEC, the luteal phase increased the RE of ZIP 6 (P ≤ 0.05), decreased the RE of ZnT9 (P ≤ 0.05), and did not modify the RE of ZIP14 (P > 0.05) compared to the follicular phase. In conclusion, the study reveals differences in the gene expression of ZIP6, ZIP14, and ZnT9 according to the estrous cycle phase in ex vivo samples of bovine COC and OEC.

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发情周期对牛积液-卵母细胞复合体和输卵管上皮细胞中锌转运体表达的影响

在发情周期的黄体期和卵泡期，精原细胞-卵母细胞复合体（COC）和输卵管上皮细胞（OEC）会发生显著的生理和形态变化。维持适当的锌（Zn）平衡对体细胞和哺乳动物生殖细胞都至关重要。本研究旨在评估发情期（黄体期或卵泡期）对牛 COC 和 OEC（BOEC）中锌转运体表达的影响。研究分析了黄体期或卵泡期奶牛COC和BOEC中锌转运体Slc39a6 (ZIP6)、Slc39a8 (ZIP8)、Slc39a14 (ZIP14)、Slc30a3 (ZnT3)、Slc30a7 (ZnT7)和Slc30a9 (ZnT9)的表达情况。对 COC 和 BOEC 中 ZIP6、ZIP14 和 ZnT9 的基因表达进行了量化。由于 mRNA 水平较低（COC 和 BOEC 中的 ZIP8 和 ZnT3；BOEC 中的 ZnT7）或无表达（COC 中的 ZnT7），其余转运体的基因表达无法量化。在 COC 中，所有三种转运体在黄体期的相对表达量（RE）均高于卵泡期（P ≤ 0.05）。在 BOEC 中，与卵泡期相比，黄体期 ZIP 6 的相对表达量增加（P ≤ 0.05），ZnT9 的相对表达量减少（P ≤ 0.05），ZIP14 的相对表达量没有变化（P > 0.05）。总之，本研究揭示了牛COC和OEC体外样本中ZIP6、ZIP14和ZnT9基因表达随发情周期阶段的不同而存在差异。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.