Anti-ageing mechanism of topical bioactive ingredient composition on skin based on network pharmacology.

IF 2.7 4区医学 Q2 DERMATOLOGY International Journal of Cosmetic Science Pub Date : 2024-09-09 DOI:10.1111/ics.13005

Wang Feifei, Su Wenrou, Sun Jinyue, Du Qiaochu, Li Jingjing, Liu Jin, Li Junxiang, Li Xuhui, Lin Xiao, He Congfen

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Abstract

Objective: To elucidate the anti-ageing mechanism of the combination of eight ingredients on the skin from a multidimensional view of the skin.

Methods: The target pathway mechanisms of composition to delay skin ageing were investigated by a network pharmacology approach and experimentally validated at three levels: epidermal, dermal, and tissue.

Results: We identified 24 statistically significant skin ageing-related pathways, encompassing crucial processes such as epidermal barrier repair, dermal collagen and elastin production, inhibition of reactive oxygen species (ROS), as well as modulation of acetylcholine and acetylcholine receptor binding. Furthermore, our in vitro experimental findings exhibited the following outcomes: the composition promotes fibroblast proliferation and the expression of barrier-related genes in the epidermis; it also stimulated the expression of collagen I, collagen III, and elastic fibre while inhibiting ROS and β-Gal levels in HDF cells within the dermis. Additionally, Spilanthol in the Acmella oleracea extract contained in the composition demonstrated neuro-relaxing activity in Zebrafish embryo, suggesting its potential as an anti-wrinkle ingredient at the hypodermis level.

Conclusions: In vitro experiments validated the anti-ageing mechanism of composition at multiple skin levels. This framework can be extended to unravel the functional mechanisms of other clinically validated compositions, including traditional folk recipes utilized in cosmeceuticals.

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基于网络药理学的皮肤外用生物活性成分的抗衰老机理。

目的从皮肤的多维视角阐明八种成分组合对皮肤的抗衰老机制：方法：通过网络药理学方法研究成分延缓皮肤衰老的目标通路机制，并在表皮、真皮和组织三个层面进行实验验证：结果：我们发现了 24 条具有统计学意义的皮肤老化相关通路，其中包括表皮屏障修复、真皮胶原蛋白和弹性蛋白生成、活性氧（ROS）抑制以及乙酰胆碱和乙酰胆碱受体结合调节等关键过程。此外，我们的体外实验结果表明：该成分能促进表皮中成纤维细胞的增殖和屏障相关基因的表达；还能刺激胶原蛋白 I、胶原蛋白 III 和弹性纤维的表达，同时抑制 ROS 和真皮中 HDF 细胞中的β-Gal 水平。此外，组合物中含有的黑醋栗提取物中的斯皮兰醇在斑马鱼胚胎中显示出神经放松活性，这表明它具有在真皮下层抗皱的潜力：结论：体外实验验证了该成分在多个皮肤层面的抗衰老机制。结论：体外实验验证了该成分在多个皮肤层面的抗衰老机制，这一框架可扩展用于揭示其他经临床验证的成分的功能机制，包括用于药妆的传统民间配方。

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来源期刊

International Journal of Cosmetic Science DERMATOLOGY-

CiteScore

4.60

自引率

4.30%

发文量

期刊介绍： The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.