LC–MS/MS-QTOF analysis of Anodendron parviflorum (Roxb.) leaves extract and exploring its antioxidant, antimicrobial, and cytotoxic potential

IF 3.4 Q2 PHARMACOLOGY & PHARMACY Future Journal of Pharmaceutical Sciences Pub Date : 2024-09-10 DOI:10.1186/s43094-024-00695-1

Arunagiri Sharmila, Chinnadurai Immanuel Selvaraj

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Abstract

Background

Traditional medicine claims that Anodendron parviflorum has benefits for treating various human diseases. The present study seeks to understand better the phytochemical and LC–MS/MS-QTOF profiling of A. parviflorum's ethanolic extract and to investigate the properties of the different solvents of A. parviflorum for anti-inflammatory, antioxidant, antimicrobial, toxicity, and cytotoxic effects.

Results

The quantitative methods exhibited higher total phenolics (327.16 ± 2.4 mg GAE/g dw), total flavonoid (109.82 ± 1.9 mg QE/g dw), and total alkaloid (14.13 ± 0.09%) content in ethanol extract. In contrast, a higher total extraction value (22.8 ± 0.6%) and total terpenoid (57.23 ± 0.06 mg LL/g dw) content was shown in the methanol extract of A. parviflorum. LC–MS/MS-QTOF analysis of its ethanolic extract revealed a notable occurrence of phenols and flavonoids. The ethanolic extract of A. parviflorum exhibited significant antioxidant activities with lower IC₅₀ values in DPPH, phosphomolybdenum, and metal chelating and reducing power assay. The methanolic extract of A. parviflorum had the more significant anti-inflammatory property (94.55 ± 0.1%) in the bovine serum albumin assay. The extracts also demonstrated a higher inhibition zone against pathogenic bacteria. The ethanolic extract of A. parviflorum demonstrated substantial cytotoxicity against A549 cells.

Conclusion

Consequently, these findings validate the use of A. parviflorum in traditional medical practices due to its bioactive compounds, which may have potential therapeutic value in various biomedical applications.

Highlights

A. parviflorum leaves extract showed the presence of significant levels of alkaloids, flavonoids, phenols, and terpenoids.
LC–MS/MS-QTOF analysis revealed notable bioactive compounds of A. parviflorum.
The different extracts showed remarkable antioxidant and antimicrobial activities.
The extracts exhibited less hemolytic and higher thrombolytic activities.
It demonstrated significant cytotoxicity against A549 cell lines.

Graphical abstract

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LC-MS/MS-QTOF分析Anodendron parviflorum（Roxb.）叶提取物并探索其抗氧化、抗菌和细胞毒性潜力

背景传统医学认为，杜鹃花（Anodendron parviflorum）具有治疗各种人类疾病的功效。本研究旨在更好地了解侧柏叶乙醇提取物的植物化学成分和 LC-MS/MS-QTOF 分析，并研究侧柏叶不同溶剂的抗炎、抗氧化、抗菌、毒性和细胞毒性作用。结果定量方法显示，乙醇提取物中的总酚（327.16 ± 2.4 mg GAE/g dw）、总黄酮（109.82 ± 1.9 mg QE/g dw）和总生物碱（14.13 ± 0.09%）含量较高。相反，甲醇提取物中的总萃取值（22.8 ± 0.6%）和总萜类化合物（57.23 ± 0.06 mg LL/g dw）含量较高。对其乙醇提取物进行的 LC-MS/MS-QTOF 分析显示，酚类和黄酮类化合物的含量显著增加。在 DPPH、磷钼、金属螯合和还原力检测中，A. parviflorum 的乙醇提取物具有显著的抗氧化活性，其 IC50 值较低。在牛血清白蛋白试验中，西番莲甲醇提取物具有更明显的抗炎特性（94.55 ± 0.1%）。这些提取物对病原菌的抑制面积也更大。A. parviflorum 的乙醇提取物对 A549 细胞具有很强的细胞毒性。因此，这些发现验证了 A. parviflorum 在传统医学实践中的应用，因为其生物活性化合物可能在各种生物医学应用中具有潜在的治疗价值。 LC-MS/MS-QTOF 分析揭示了 A. parviflorum 的重要生物活性化合物。不同的萃取物显示出显著的抗氧化和抗菌活性。这些提取物具有较低的溶血活性和较高的溶栓活性。它对 A549 细胞系具有明显的细胞毒性。图表摘要

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Future Journal of Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-

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审稿时长

23 weeks

期刊介绍： Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.