Application of Cytochrome P450 Monooxygenases in Plant for Rapid Detection of 2,3,7,8 Tetrachlorodibenzodioxin in the Contaminated Sites

IF 2.6 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES International Journal of Environmental Research Pub Date : 2024-08-26 DOI:10.1007/s41742-024-00640-3
Ho Man Leung, Ka Chun Sung, Lai Yan Woo, Wing Yin Mo, Kwai Chung Cheung, Chi Kin Au, Ken Kin Lam Yung, Wai Chin Li
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Abstract

The function of Cytochrome (CYP) P450 in plants to enhance detoxification of herbicide metabolism is well-known. However, the knowledge of gene quantification for detecting and detoxifying pollutants and other toxicants by an indigenous plant growing in a contaminated site is limited. The objective of this research is to evaluate the potential of detecting or degrading 2,3,7,8 Tetrachlorodibenzodioxin (TCDD) in soil using a native plant growing in a contaminated site via the gene expression of Cytochrome P450 monooxygenases (P450s) method. The novelty of this research is that P450s in native plants possibly acts as a bioindicator on contaminated land by increasing its gene expression levels induced by the presence of TCDD. In seedling toxicity test and cytochrome enzyme activity test, a significant difference in the root length (range of value: 580.2–799.2 mm) and enzyme activity (range of value: 31.2–82.3 nmolmin−1 g−1 total protein) of such indigenous plant was found in 10 µg/L TCDD treatment when compared to other treatments. 13- and 20-fold levels of gene expression in CYP71C1 and CYP79A61 of the plant growing in a contaminated site were found after 10 µg/L TCDD treatment. The results revealed that such indigenous plant is sensitive to the detection of such persistent organic pollutant in the field site and involves gene expression change facilitated by a plant‒microbe symbiotic association. The current findings can provide an insight to use another option for pollution monitoring using non-standard plant models.

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应用植物中的细胞色素 P450 单氧化酶快速检测受污染场地中的 2,3,7,8 四氯二苯并二噁英
众所周知,植物中的细胞色素(CYP)P450 具有增强除草剂代谢解毒的功能。然而,对于生长在受污染地区的本地植物检测和解毒污染物及其他有毒物质的基因定量知识却很有限。本研究的目的是通过细胞色素 P450 单氧化酶(P450s)基因表达的方法,评估一种生长在受污染地区的本地植物检测或降解土壤中 2,3,7,8-四氯二苯并对二恶英(TCDD)的潜力。这项研究的新颖之处在于,本地植物中的 P450s 可能会在 TCDD 存在的诱导下提高基因表达水平,从而成为受污染土地的生物指示剂。在幼苗毒性测试和细胞色素酶活性测试中发现,与其他处理相比,10 µg/L TCDD 处理的本地植物根长(数值范围:580.2-799.2 mm)和酶活性(数值范围:31.2-82.3 nmolmin-1 g-1 总蛋白)有显著差异。经 10 µg/L TCDD 处理后,生长在受污染地区的植物的 CYP71C1 和 CYP79A61 基因表达量分别增加了 13 倍和 20 倍。结果表明,这种本地植物对田间地头检测到的这种持久性有机污染物很敏感,并在植物与微生物共生关系的促进下发生了基因表达变化。目前的研究结果为利用非标准植物模型进行污染监测提供了另一种选择。
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来源期刊
CiteScore
5.40
自引率
0.00%
发文量
104
审稿时长
1.7 months
期刊介绍: International Journal of Environmental Research is a multidisciplinary journal concerned with all aspects of environment. In pursuit of these, environmentalist disciplines are invited to contribute their knowledge and experience. International Journal of Environmental Research publishes original research papers, research notes and reviews across the broad field of environment. These include but are not limited to environmental science, environmental engineering, environmental management and planning and environmental design, urban and regional landscape design and natural disaster management. Thus high quality research papers or reviews dealing with any aspect of environment are welcomed. Papers may be theoretical, interpretative or experimental.
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