Punica granatum L. peel extract protects diabetic nephropathy by activating the Nrf-2/HO-1 pathway

IF 3.1 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Acta Diabetologica Pub Date : 2024-09-11 DOI:10.1007/s00592-024-02371-5

Betul Apaydin Yildirim, Tuba Dogan, Esra Aktas Senocak, Serkan Yildirim, Saban Kordali, Fatih Yildirim

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Abstract

Diabetes raises cardiovascular morbidity and mortality worldwide and causes retinopathy, neuropathy, and nephropathy. Punica granatum L. (Pomegranate) is a fruit that has been used for its medicinal properties in various cultures. This article aims to investigate the antioxidant, anti-inflammatory, anti-apoptotic activity of Punica granatum L. peel ethanol extract (PGE) and its efficacy on NF-κB and Nrf-2/HO-1 signaling pathways in kidney tissue of rats with streptozotocin-induced diabetes. Single dose STZ 60 mg/kg/i.p. rats were given to induce diabetes and blood glucose measurements were taken 7 days later. PGE 10 mg/kg/p.o. administered to the treatment groups for 20 days. Blood, kidney, and pancreas samples taken from anesthetized rats were analyzed biochemically and histopathologically. It was observed that STZ increased the levels of urea, uric acid and creatine in the blood, while PGE significantly decreased these parameters. The diabetic group had higher MDA and lower renal tissue GSH level, CAT, GPx, and SOD activity than the control group. STZ also enhanced inflammation, apoptosis, Bax, Caspase-3, and NF-κB expression, and decreased Bcl-2, HO-1, and Nrf-2 expression. Experimental results showed that PGE has the potential to alleviate the harmful effects on the kidney and pancreas by altering the mentioned parameters in diabetic rats.

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石榴皮提取物通过激活 Nrf-2/HO-1 通路保护糖尿病肾病

糖尿病在全球范围内增加了心血管疾病的发病率和死亡率，并导致视网膜病变、神经病变和肾病。石榴（Punica granatum L.）是一种水果，在各种文化中都有药用价值。本文旨在研究石榴皮乙醇提取物（PGE）的抗氧化、抗炎和抗细胞凋亡活性及其对链脲佐菌素诱导糖尿病大鼠肾组织中 NF-κB 和 Nrf-2/HO-1 信号通路的影响。给大鼠注射单剂量 STZ 60 mg/kg/i.p.诱导糖尿病，7 天后测量血糖。给治疗组注射 PGE 10 mg/kg/p.o.，共 20 天。对麻醉大鼠的血液、肾脏和胰腺样本进行生化和组织病理学分析。结果发现，STZ 增加了血液中尿素、尿酸和肌酸的含量，而 PGE 则显著降低了这些参数。与对照组相比，糖尿病组的 MDA 水平更高，肾组织 GSH 水平、CAT、GPx 和 SOD 活性更低。STZ 还能增强炎症、细胞凋亡、Bax、Caspase-3 和 NF-κB 的表达，降低 Bcl-2、HO-1 和 Nrf-2 的表达。实验结果表明，PGE 有可能通过改变糖尿病大鼠的上述参数来减轻对肾脏和胰腺的危害。

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来源期刊

Acta Diabetologica 医学-内分泌学与代谢

CiteScore

7.30

自引率

2.60%

发文量

180

审稿时长

2 months

期刊介绍： Acta Diabetologica is a journal that publishes reports of experimental and clinical research on diabetes mellitus and related metabolic diseases. Original contributions on biochemical, physiological, pathophysiological and clinical aspects of research on diabetes and metabolic diseases are welcome. Reports are published in the form of original articles, short communications and letters to the editor. Invited reviews and editorials are also published. A Methodology forum, which publishes contributions on methodological aspects of diabetes in vivo and in vitro, is also available. The Editor-in-chief will be pleased to consider articles describing new techniques (e.g., new transplantation methods, metabolic models), of innovative importance in the field of diabetes/metabolism. Finally, workshop reports are also welcome in Acta Diabetologica.