{"title":"Isolation and characterization of forty-two polymorphic simple sequence repeat markers from Mastacembelus armatus (Symbranchiformes: Mastacembelidae)","authors":"Laifu Luo, Liqin Ji, Congcong Wu, Wei Li, Xiaoli Liu, Xiaoyou Hong, Xinping Zhu, Yijun Ni, Jiansong Li, Chen Chen, Jiping Zhang","doi":"10.1007/s12686-024-01365-3","DOIUrl":null,"url":null,"abstract":"<p>A total of 42 polymorphic simple sequence repeat (SSR) markers were developed and characterized from genome sequences for the “zig-zag eel”, <i>Mastacembelus armatus</i>, to safeguard its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 35 individuals. The number of alleles per locus ranged from two to fourteen. The observed heterozygosity and expected heterozygosity varied from 0.031 to 0.813, and from 0.091 to 0.852, respectively. Three SSR loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These loci have been characterized for the first time and will be useful for genetic conservation and marker-assisted breeding of <i>M. armatus.</i></p>","PeriodicalId":10625,"journal":{"name":"Conservation Genetics Resources","volume":"25 1","pages":""},"PeriodicalIF":0.9000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Conservation Genetics Resources","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s12686-024-01365-3","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIODIVERSITY CONSERVATION","Score":null,"Total":0}
引用次数: 0
Abstract
A total of 42 polymorphic simple sequence repeat (SSR) markers were developed and characterized from genome sequences for the “zig-zag eel”, Mastacembelus armatus, to safeguard its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 35 individuals. The number of alleles per locus ranged from two to fourteen. The observed heterozygosity and expected heterozygosity varied from 0.031 to 0.813, and from 0.091 to 0.852, respectively. Three SSR loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These loci have been characterized for the first time and will be useful for genetic conservation and marker-assisted breeding of M. armatus.
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Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.
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