Lucia Urbanová, Jana Bilčíková, Dagmar Moravčíková, Jana Žiarovská
{"title":"Natural Variability of Genomic Sequences of Mal d 1 Allergen in Apples as Revealed by Restriction Profiles and Homolog Polymorphism","authors":"Lucia Urbanová, Jana Bilčíková, Dagmar Moravčíková, Jana Žiarovská","doi":"10.3390/agronomy14092056","DOIUrl":null,"url":null,"abstract":"Apples are a popular fruit worldwide, with many health and nutritional benefits. However, this fruit is also among those that, particularly in Central and Northern Europe, are allergenic due to the Mal d 1 allergen. Mal d 1 is a homologous allergen to Bet v 1—the main pollen allergen of birch. In this study, two different approaches were used to identify the natural length polymorphism of Bet v 1 homologs in apple varieties, with the aim of characterizing their effectiveness. BBAP (Bet v 1 based amplified polymorphism) and RFLP (restriction fragments length polymorphism) profiles were characterized and compared. RFLP analysis recognizes the genetic diversity of M. domestica Mal d 1 sequences at a relatively low level. In BBAP profiles, the genetic dissimilarity was up to 50%, which appears suitable for intraspecific fingerprinting and serves as an additional method for RFLP analysis. RFLP analysis was able to distinguish some varieties that BBAP could not, such as Sonet.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"13 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agronomy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/agronomy14092056","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Apples are a popular fruit worldwide, with many health and nutritional benefits. However, this fruit is also among those that, particularly in Central and Northern Europe, are allergenic due to the Mal d 1 allergen. Mal d 1 is a homologous allergen to Bet v 1—the main pollen allergen of birch. In this study, two different approaches were used to identify the natural length polymorphism of Bet v 1 homologs in apple varieties, with the aim of characterizing their effectiveness. BBAP (Bet v 1 based amplified polymorphism) and RFLP (restriction fragments length polymorphism) profiles were characterized and compared. RFLP analysis recognizes the genetic diversity of M. domestica Mal d 1 sequences at a relatively low level. In BBAP profiles, the genetic dissimilarity was up to 50%, which appears suitable for intraspecific fingerprinting and serves as an additional method for RFLP analysis. RFLP analysis was able to distinguish some varieties that BBAP could not, such as Sonet.
苹果是一种风靡全球的水果，对健康和营养有很多益处。然而，这种水果也属于因 Mal d 1 过敏原而导致过敏的水果，尤其是在中欧和北欧。Mal d 1 与桦树的主要花粉过敏原 Bet v 1 是同源过敏原。本研究采用了两种不同的方法来鉴定苹果品种中 Bet v 1 同源物的天然长度多态性，以确定其有效性。研究人员对 BBAP（基于 Bet v 1 的扩增多态性）和 RFLP（限制性片段长度多态性）图谱进行了鉴定和比较。RFLP 分析表明，M. domestica Mal d 1 序列的遗传多样性水平相对较低。在 BBAP 图谱中，遗传相似度高达 50%，这似乎适合种内指纹图谱分析，也可作为 RFLP 分析的一种补充方法。RFLP 分析能够区分一些 BBAP 无法区分的品种，如 Sonet。
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