Therapeutic effects of focused ultrasound on vulvar squamous intraepithelial lesions in rat.

IF 3 3区 医学 Q2 ONCOLOGY International Journal of Hyperthermia Pub Date : 2024-09-10 DOI:10.1080/02656736.2024.2401417
Yijin Fan,Xi Wang,Yao Liu,Huajun Tang,Chengzhi Li
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Abstract

OBJECTIVE In this study, we established a Sprague-Dawley rat model of vulvar squamous intraepithelial lesions and investigated the impact of focused ultrasound on the expression of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and mutant type p53 (mtp53) in the vulvar skin of rats with low-grade squamous intraepithelial lesions (LSIL). MATERIALS AND METHODS The vulvar skin of 60 rats was treated with dimethylbenzanthracene (DMBA) and mechanical irritation three times a week for 14 weeks. Rats with LSIL were randomly allocated into the experimental group or the control group. The experimental group was treated with focused ultrasound, while the control group received sham treatment. RESULTS After 14 weeks treatment of DMBA combined with mechanical irritation, LSIL were observed in 44 (73.33%) rats, and high-grade squamous intraepithelial lesions (HSIL) were observed in 14 (23.33%) rats. 90.91% (20/22) of rats showed normal pathology and 9.09% (2/22) of rats exhibited LSIL in the experimental group at four weeks after focused ultrasound treatment. 22.73% (5/22) of rats exhibited LSIL, 77.27% (17/22) of rats progressed to HSIL in the control group. Compared with the control-group rats, the levels of HIF-1α, VEGF and mtp53 were significantly decreased in experimental-group rats (p < 0.05). CONCLUSIONS These results indicate that DMBA combined with mechanical irritation can induce vulvar squamous intraepithelial lesion in SD rats. Focused ultrasound can treat LSIL safely and effectively, prevent the progression of vulvar lesions, and improve the microenvironment of vulvar tissues by decreasing the localized expression of HIF-1α, VEGF, and mtp53 in rats.
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聚焦超声对大鼠外阴鳞状上皮内病变的治疗作用
目的 本研究建立了 Sprague-Dawley 大鼠外阴鳞状上皮内病变模型,并探讨了聚焦超声对低级鳞状上皮内病变(LSIL)大鼠外阴皮肤中缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)和突变型 p53(mtp53)表达的影响。材料与方法对 60 只大鼠的外阴皮肤进行二甲基苯并蒽 (DMBA) 和机械刺激处理,每周三次,持续 14 周。患有 LSIL 的大鼠被随机分配到实验组或对照组。结果经过 14 周的 DMBA 联合机械刺激治疗后,44 只(73.33%)大鼠观察到 LSIL,14 只(23.33%)大鼠观察到高级别鳞状上皮内病变(HSIL)。聚焦超声治疗四周后,90.91%(20/22)的大鼠病理结果正常,9.09%(2/22)的大鼠出现 LSIL。对照组有 22.73%(5/22)的大鼠出现 LSIL,77.27%(17/22)的大鼠发展为 HSIL。与对照组大鼠相比,实验组大鼠的 HIF-1α、VEGF 和 mtp53 水平显著下降(P < 0.05)。聚焦超声可安全有效地治疗外阴鳞状上皮内瘤变(LSIL),防止外阴病变进展,并通过降低大鼠局部 HIF-1α、VEGF 和 mtp53 的表达改善外阴组织的微环境。
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来源期刊
CiteScore
5.90
自引率
12.90%
发文量
153
审稿时长
6-12 weeks
期刊介绍: The International Journal of Hyperthermia
期刊最新文献
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