Characterization of testicular embryonic-type neuroectodermal tumor and embryonic-type neuroectodermal tissue admixed with mature neuro-glial tissue using a broad immunohistochemical panel

IF 3.4 3区医学 Q1 PATHOLOGY Virchows Archiv Pub Date : 2024-09-11 DOI:10.1007/s00428-024-03911-8

Luisa Di Sciascio, Agnese Orsatti, Francesca Ambrosi, Eugenia Franchini, Francesco Massari, Veronica Mollica, Federico Mineo Bianchi, Maurizio Colecchia, Antonio De Leo, Andres Martin Acosta, João Lobo, Michelangelo Fiorentino, Costantino Ricci

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Abstract

Embryonic-type neuroectodermal tumor (ENT) is a somatic-type malignancy characterized by overgrowth of embryonic-type neuroectodermal tissue (EtNT). In germ cell tumors, EtNT is frequently intermingled with other components that may exhibit significant morphologic overlap [mature neuro-glial tissue (MNGT), nephroblastomatous tissues, and primitive endodermal-type glands]. Therefore, the quantification of EtNT (crucial for the diagnosis of ENT) can be challenging. In this study, we investigated the immunohistochemical profile of ENT, EtNT, and MNGT using a broad immunohistochemical panel. We found that SOX2 was the most sensitive marker for EtNT (100%), but it also stained MNGT (28.6%). GFAP and S100 were relatively sensitive (71.4%) and highly specific (GFAP 100%, S100 85.8%) for MNGT, whereas synaptophysin stained both. Combining our results with those of previous studies, we propose that a combination of SOX11, SOX2, GFAP, S100, AFP, villin, CDX2, PAX8, and nuclear WT1 may help to identify and quantify EtNT in germ cell tumors.

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使用广泛的免疫组化面板鉴定睾丸胚胎型神经外胚层肿瘤和胚胎型神经外胚层组织与成熟神经胶质组织混合的特征

胚胎型神经外胚层肿瘤（ENT）是一种以胚胎型神经外胚层组织（EtNT）过度生长为特征的体细胞型恶性肿瘤。在生殖细胞瘤中，EtNT 经常与其他成分（成熟神经胶质组织 (MNGT)、肾母细胞瘤组织和原始内胚层型腺体）混杂在一起，这些成分在形态上可能有明显的重叠。因此，EtNT 的定量（对耳鼻喉科诊断至关重要）可能具有挑战性。在本研究中，我们使用一个广泛的免疫组化面板研究了耳鼻咽喉癌、EtNT 和 MNGT 的免疫组化特征。我们发现，SOX2 是对 EtNT 最敏感的标记物（100%），但它也能对 MNGT 进行染色（28.6%）。GFAP 和 S100 对 MNGT 相对敏感（71.4%），特异性高（GFAP 100%，S100 85.8%），而突触素对两者均有染色。将我们的研究结果与之前的研究结果相结合，我们认为将 SOX11、SOX2、GFAP、S100、AFP、villin、CDX2、PAX8 和核 WT1 结合在一起可能有助于识别和量化生殖细胞肿瘤中的 EtNT。

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来源期刊

Virchows Archiv 医学-病理学

CiteScore

7.40

自引率

2.90%

发文量

204

审稿时长

4-8 weeks

期刊介绍： Manuscripts of original studies reinforcing the evidence base of modern diagnostic pathology, using immunocytochemical, molecular and ultrastructural techniques, will be welcomed. In addition, papers on critical evaluation of diagnostic criteria but also broadsheets and guidelines with a solid evidence base will be considered. Consideration will also be given to reports of work in other fields relevant to the understanding of human pathology as well as manuscripts on the application of new methods and techniques in pathology. Submission of purely experimental articles is discouraged but manuscripts on experimental work applicable to diagnostic pathology are welcomed. Biomarker studies are welcomed but need to abide by strict rules (e.g. REMARK) of adequate sample size and relevant marker choice. Single marker studies on limited patient series without validated application will as a rule not be considered. Case reports will only be considered when they provide substantial new information with an impact on understanding disease or diagnostic practice.