The ERF transcription factor SlERF7 promotes UV-C-induced biosynthesis of phenolic compounds in tomato

IF 3.9 2区农林科学 Q1 HORTICULTURE Scientia Horticulturae Pub Date : 2024-09-13 DOI:10.1016/j.scienta.2024.113643

Chenchen Wu , Chuanlong Men , Ling Yan , Jing Zhang , Yaqian Wang , Miao Chen , Changhong Liu , Lei Zheng

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Abstract

In this study, the molecular pathways of SlERF7 in regulating the synthesis and accumulation of phenolic compounds induced by postharvest UV-C in tomato fruit was deeply investigated by constructing transgenic plants. The findings revealed a pronounced induction of phenolic compounds accumulation following UV-C irradiation, coupled with heightened activities of PAL, 4CL, C4H, CHS, and CHI enzymes, alongside upregulated expression levels of SlPAL5, SlC4H, Sl4CL, SlCHS2, and SlCHI within SlERF7 overexpressed fruit. Conversely, SlERF7 knockout fruit exhibited diminished levels of phenolic compounds, enzyme activities, and gene expression after UV-C irradiation. Furthermore, subcellular localization analysis showed that SlERF7 was localized in the nucleus. Dual luciferase assay and electrophoretic mobility shift assay (EMSA) indicated that SlERF7 directly bound to the GCC-box in the SlPAL5 promoter and activated its transcriptional activity. Therefore, it was confirmed that SlERF7 might positively promote UV-C-induced phenolic biosynthesis in postharvest tomato fruit by targeting the promoter of SlPAL5.

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ERF转录因子SlERF7促进紫外线-C诱导的番茄酚类化合物的生物合成

本研究通过构建转基因植株，深入研究了SlERF7调控采后紫外线-C诱导番茄果实中酚类化合物合成和积累的分子途径。研究结果表明，紫外线-C照射后，番茄果实中的PAL、4CL、C4H、CHS和CHI酶活性增强，同时SlPAL5、SlC4H、Sl4CL、SlCHS2和SlCHI在SlERF7过表达的果实中表达水平升高，从而明显诱导了酚类化合物的积累。相反，SlERF7基因敲除果实在紫外线-C照射后表现出酚类化合物、酶活性和基因表达水平降低。此外，亚细胞定位分析表明，SlERF7 定位于细胞核中。双荧光素酶试验和电泳迁移试验（EMSA）表明，SlERF7直接与SlPAL5启动子中的GCC-box结合并激活其转录活性。因此，证实 SlERF7 可通过靶向 SlPAL5 启动子，积极促进 UV-C 诱导的番茄采后果实中的酚类生物合成。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.