{"title":"Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection","authors":"H. Yahaya, Y. K. Cheah, H. Y. Chee, L. T. L. Than","doi":"10.1007/s10096-024-04934-4","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Purpose</h3><p>Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of <i>C. glabrata</i>.</p><h3 data-test=\"abstract-sub-heading\">Methodology</h3><p>The specific LAMP primers for <i>C. glabrata</i> detection were designed and evaluated.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The LAMP assay accurately detected <i>C. glabrata</i> with no cross-reactivity with other <i>Candida</i> species.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>The developed molecular method would be a promising tool in the management of invasive candidiasis.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":"6 1","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Clinical Microbiology & Infectious Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10096-024-04934-4","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose
Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata.
Methodology
The specific LAMP primers for C. glabrata detection were designed and evaluated.
Results
The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.
Conclusion
The developed molecular method would be a promising tool in the management of invasive candidiasis.
期刊介绍：
EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
