PdPtCu mesoporous nanocube-based electrochemical sandwich immunosensor for detection of HIV-p24

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioelectrochemistry Pub Date : 2024-09-18 DOI:10.1016/j.bioelechem.2024.108819

Xin Li , Daobin Han , Xinmin Li , Chunjie Zhou , Bo Shen , Honglu Wei , Qian Lou , Changjin Liu , Tingmei Chen

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Abstract

The construction of simple, stable, low-cost and reproducible enzyme-free electrochemical biosensors can effectively avoid the problem of signal attenuation caused by enzyme inactivation. Hererin, we prepared a novel nanoenzymes PdPtCu mesoporous nanocubes (MNCs) to construct a label-free sandwich electrochemical immunosensor for the highly sensitivity detection of HIV-p24. PdPtCu MNCs have excellent peroxidase activity against hydrogen peroxide (H₂O₂) due to their synergistic ternary composition, large surface area and ability to penetrate mesoporous channels. Moreover, highly conductive and biocompatible gold nanoparticles@graphene oxide (AuNPs@GO) was introduced as a substrate to modify a glassy carbon electrode (GCE). Owing to the excellent electrochemical performance of the PdPtCu MNCs and AuNPs@GO, the developed immunosensors exhibited a good linear response from 0.04 pg/mL to 100 ng/mL with a low detection limit of 20 fg/mL. In addition, the established method exhibited excellent practical performance in human serum. This novel strategy provides a promising platform for ultrasensitive detection of the HIV-p24 in the field of clinical diagnostics.

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基于 PdPtCu 介孔纳米立方体的电化学夹心免疫传感器用于检测 HIV-p24

构建简单、稳定、低成本和可重现的无酶电化学生物传感器可以有效避免酶失活导致的信号衰减问题。为此，我们制备了一种新型纳米酶PdPtCu介孔纳米立方体（MNCs），构建了一种高灵敏度检测HIV-p24的无标记夹心电化学免疫传感器。PdPtCu 介孔纳米立方体具有协同三元成分、大表面积和穿透介孔通道的能力，因此对过氧化氢（H2O2）具有优异的过氧化物酶活性。此外，还引入了高导电性和生物相容性的金纳米粒子@氧化石墨烯（AuNPs@GO）作为改性玻璃碳电极（GCE）的基底。由于 PdPtCu MNCs 和 AuNPs@GO 具有优异的电化学性能，所开发的免疫传感器在 0.04 pg/mL 至 100 ng/mL 范围内具有良好的线性响应，检测限低至 20 fg/mL。此外，所建立的方法在人血清中表现出优异的实用性。这种新型策略为临床诊断领域的 HIV-p24 超灵敏检测提供了一个前景广阔的平台。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.