Quantitative assessment of Nigella sativa and conjugated silver nanoparticles against hexavalent chromium toxic effects on sperm function

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY Toxicon Pub Date : 2024-09-12 DOI:10.1016/j.toxicon.2024.108102

Tooba Nauroze , Shaukat Ali , Shagufta Andleeb , Chaman Ara , Lubna Kanwal , Samaira Mumtaz , Muhammad Summer , Rizwan Ullah

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Abstract

Background

Infertility has been observed as one of the major issues in humans, one known risk factor is heavy metals.

Methods

The main focus of the present research was to assess the toxic effect of hexavalent chromium (Cr (VI)) on sperm and its mitigation by Nigella sativa seed extract (NS) and its conjugated silver nanoparticles (NS + NP). In the present study, we administered 1.5 mg/kg body of Cr (VI) orally in mice for 60 days routinely, to induce toxicity in testes and effect on sperm production and motility in male mice. NS and NS + NP (50 mg/kg body weight) were administered to evaluate protective action against Cr (VI). The sperm were analyzed by computer-assisted semen analysis (CASA) and chromium concentration in testicular tissue was measured via the atomic absorption spectrophotometer.

Results

The CASA analysis showed that Cr (VI) was directly linked with a decline in sperm concentration, motility, distance, velocity, straightness, and head beat frequency attributes. However, the administration of Nigella sativa seed extract and its green synthesized silver nanoparticles improved sperm concentration, motility, distance, velocity, straightness, and head beat frequency. The chromium content in the testes of Cr-exposed animals significantly increased, which negatively affected sperm parameters. However, Nigella sativa and Nigella sativa conjugated silver nanoparticles appeared to help in the removal of Cr content from testes hence improving the sperm parameters in exposed mice.

Conclusion

The decrease in Cr concentration improved sperm quality and quantity, hence, improve male fertility.

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定量评估黑麦草和共轭银纳米粒子对六价铬毒性对精子功能的影响

方法本研究的重点是评估六价铬（Cr (VI)）对精子的毒性作用，以及通过黑麦草种子提取物（NS）及其共轭银纳米粒子（NS + NP）来缓解这种作用。在本研究中，我们按常规给小鼠口服 1.5 mg/kg 体重的 Cr (VI)，连续 60 天，以诱导雄性小鼠睾丸的毒性并影响精子的生成和活力。为了评估对六价铬的保护作用，小鼠还服用了 NS 和 NS + NP（50 毫克/千克体重）。通过计算机辅助精液分析（CASA）对精子进行了分析，并通过原子吸收分光光度计测量了睾丸组织中的铬浓度。然而，服用黑升麻籽提取物及其绿色合成的银纳米粒子可提高精子浓度、活力、距离、速度、直线度和头部跳动频率。铬暴露动物睾丸中的铬含量显著增加，对精子参数产生了负面影响。然而，黑麦草和黑麦草共轭银纳米粒子似乎有助于清除睾丸中的铬含量，从而改善暴露小鼠的精子参数。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

期刊介绍： Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.