The fed-batch production of mannosylerythritol lipids by Ustilago maydis DSM 4500 from hydrophilic carbon sources.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-12-01 Epub Date: 2024-09-21 DOI:10.1007/s00449-024-03084-3

André D Valkenburg, George M Teke, Robert W M Pott, Eugéne van Rensburg

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Abstract

Glycolipids are a class of widely studied biosurfactants with excellent applicability in cosmetic and pharmaceutical formulations. This class of biosurfactants includes mannosylerythritol lipids (MELs), which have gained particular interest due to their moisturizing and healing activity for dry and damaged human skin, arising from conditions such as eczema. Traditionally, MELs have been produced by growing certain basidiomycetous yeasts on vegetable oils. However, oils are a comparatively expensive substrate, which negatively affects the economic performance of MEL production. In addition to this, vegetable oils significantly complicate the downstream processing required to produce a product with the required purity for most applications. To address these challenges, this study investigated MEL-A production exclusively from hydrophilic carbon sources by Ustilago maydis DSM 4500. By implementing a fed-batch production strategy, maximum MEL-A concentration of 0.87 g/L was achieved from glucose exclusively. Also, adding micronutrients (such as MnSO₄) to MEL-A production showed a 24.1% increase in the product titer, implying other metabolites are formed, favoring MEL production.

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Ustilago maydis DSM 4500 利用亲水性碳源批量生产甘露糖赤藓糖醇脂。

糖脂是一类经过广泛研究的生物表面活性剂，在化妆品和药物配方中具有极佳的适用性。这类生物表面活性剂包括甘露糖基季戊四醇脂（MELs），由于其对干燥和受损的人体皮肤（如湿疹）具有保湿和修复活性，因此特别受到关注。传统上，MELs 是通过在植物油上培养某些基枝酵母而产生的。然而，植物油是一种相对昂贵的基质，这对 MEL 生产的经济效益产生了负面影响。此外，植物油还大大增加了生产大多数应用所需的纯度产品所需的下游加工的复杂性。为了应对这些挑战，本研究调查了 Ustilago maydis DSM 4500 完全利用亲水性碳源生产 MEL-A的情况。通过实施喂料批量生产策略，仅从葡萄糖中就获得了 0.87 克/升的最大 MEL-A 浓度。此外，在 MEL-A 生产过程中添加微量营养素（如 MnSO4）可使产品滴度提高 24.1%，这意味着其他代谢物的形成有利于 MEL 的生产。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.