Salinirarus marinus gen. nov., sp. nov., Haloplanus salilacus sp. nov., Haloplanus pelagicus sp. nov., Haloplanus halophilus sp. nov., Haloplanus halobius sp. nov., halophilic archaea isolated from commercial coarse salts with potential as starter cultures for salt-fermented foods.

IF 2.2 4区生物学 Q3 MICROBIOLOGY Fems Microbiology Letters Pub Date : 2024-09-20 DOI:10.1093/femsle/fnae075

Qing-Ke Zhang, Ling-Rui Zhu, Xin-Yue Dong, Xiao-Yan Yang, Jing Hou, Heng-Lin Cui

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Abstract

Five halophilic archaeal strains, XH8T, CK5-1T, GDY1T, HW8-1T, and XH21T, were isolated from commercial coarse salt produced in different regions of China. Their 16S rRNA and rpoB' gene sequences indicated that four of the strains (CK5-1T, GDY1T, HW8-1T, and XH21T) represent distinct species within the genus Haloplanus (family Haloferacaceae), while strain XH8T represents a novel genus within the same family. These assignments were supported by phylogenetic and phylogenomic analyses, which showed that strains CK5-1T, GDY1T, HW8-1T, and XH21T cluster with the current species of the genus Haloplanus, while strain XH8T forms a separate branch from the genus Haloplanus. The digital DNA-DNA hybridization and average amino acid identity (AAI) values among these four strains and the current members of the genus Haloplanus were 23.1-35.2% and 75.9-83.8%, respectively; and those values between strain XH8T and other genera in the family Haloferacaceae were 18.8-33.6% and 59.8-66.6%, respectively, much lower than the threshold values for species demarcation. Strain XH8T may represent a novel genus of the family Haloferacaceae according to the cut-off value of AAI (≤72.1%) proposed to differentiate genera within the family Haloferacaceae. These five strains could be distinguished from the related species according to differential phenotypic characteristics. Based on these results, it is proposed that strain XH8T represents a novel genus within the family Haloferacaceae, and strains CK5-1T, GDY1T, HW8-1T, and XH21T represent four novel species of the genus Haloplanus, respectively. Additionally, these five strains possess genes encoding enzymes critical for the fermentation process in salt-fermented foods, indicating their potential as starter cultures for these applications.

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Salinirarus marinus gen.nov.，sp.nov.，Haloplanus salilacus sp.nov.，Haloplanus pelagicus sp.nov.，Haloplanus halophilus sp.nov.，Haloplanus halobius sp.nov.，从商业粗盐中分离的嗜卤古细菌，具有作为盐发酵食品启动培养物的潜力。

从中国不同地区生产的商品粗盐中分离出了五株嗜卤古细菌（XH8T、CK5-1T、GDY1T、HW8-1T和XH21T）。它们的 16S rRNA 和 rpoB'基因序列表明，其中四个菌株（CK5-1T、GDY1T、HW8-1T 和 XH21T）代表卤虫科卤虫属中的不同种，而菌株 XH8T 则代表同科中的一个新属。系统发生学和系统发生组分析表明，菌株 CK5-1T、GDY1T、HW8-1T 和 XH21T 与 Haloplanus 属的现有物种聚类，而菌株 XH8T 则从 Haloplanus 属中分离出来。这四株菌株与卤虫属现有成员之间的数字 DNA-DNA 杂交值和平均氨基酸同一性（AAI）值分别为 23.1%-35.2%和 75.9%-83.8%；而 XH8T 菌株与卤虫科其他属之间的数字 DNA-DNA 杂交值和平均氨基酸同一性（AAI）值分别为 18.8%-33.6%和 59.8%-66.6%，远低于物种划分的临界值。根据 Haloferacaceae 科属区分的 AAI 临界值（≤72.1%），XH8T 菌株可能代表 Haloferacaceae 科的一个新属。根据不同的表型特征，这五个菌株可与相关种区分开来。根据这些结果，提出菌株 XH8T 代表 Haloferacaceae 中的一个新属，菌株 CK5-1T、GDY1T、HW8-1T 和 XH21T 分别代表 Haloplanus 属的四个新种。此外，这五种菌株还具有编码盐发酵食品发酵过程中关键酶的基因，这表明它们具有作为盐发酵食品启动培养物的潜力。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.