Protocol for the quantitative analysis of images retrieved by multiplex immunofluorescence staining to allow cell type-specific spatial phenotyping of markers of interest in the human placenta.
{"title":"Protocol for the quantitative analysis of images retrieved by multiplex immunofluorescence staining to allow cell type-specific spatial phenotyping of markers of interest in the human placenta.","authors":"Theresa Forndran, Silke Große, Gina Weber, Lara Hausdorf, Dagmar Samsel, Alexander Berndt, Nikolaus Gaßler, Tanja Groten","doi":"10.1016/j.placenta.2024.09.012","DOIUrl":null,"url":null,"abstract":"<p><p>In contrast to other tissues, the placenta consists of numerous functionally different cell types, distributed in a markedly dissimilar manner within one placenta and between different cases. To evaluate pathology-specific changes in cell phenotype and expression of molecular markers it is important to establish a multi staining method combining immunohistological identification of the cell type with staining of proteins of interest. We successfully established a protocol for a 6-plex antibody panel for multiplex immunofluorescence. Here, we report the staining protocol and the establishment of the quantification algorithm we developed.</p>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Placenta","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.placenta.2024.09.012","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In contrast to other tissues, the placenta consists of numerous functionally different cell types, distributed in a markedly dissimilar manner within one placenta and between different cases. To evaluate pathology-specific changes in cell phenotype and expression of molecular markers it is important to establish a multi staining method combining immunohistological identification of the cell type with staining of proteins of interest. We successfully established a protocol for a 6-plex antibody panel for multiplex immunofluorescence. Here, we report the staining protocol and the establishment of the quantification algorithm we developed.
期刊介绍：
Placenta publishes high-quality original articles and invited topical reviews on all aspects of human and animal placentation, and the interactions between the mother, the placenta and fetal development. Topics covered include evolution, development, genetics and epigenetics, stem cells, metabolism, transport, immunology, pathology, pharmacology, cell and molecular biology, and developmental programming. The Editors welcome studies on implantation and the endometrium, comparative placentation, the uterine and umbilical circulations, the relationship between fetal and placental development, clinical aspects of altered placental development or function, the placental membranes, the influence of paternal factors on placental development or function, and the assessment of biomarkers of placental disorders.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
