Target-promoted activation of DNAzyme walker for in situ assembly of hemin/G-quadruplex nanowires enable ultrasensitive and label-free electrochemical myocardial microRNA assay.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-19 DOI:10.1016/j.talanta.2024.126923

Tengteng Shan, Lingrong Cui, Huimin Zhang, Kaiqin Li, Jianmei Yang, Yan Zhao, Yun Xiang, Ruo Yuan

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Abstract

The concentration elevation of myocardial microRNA (miRNA) biomarker is associated with the pathogenic process of acute myocardial infarction (AMI), and sensitive quantification of myocardial miRNA biomarker plays an important role for early AMI diagnosis and its treatment. In response, this work describes an ultrasensitive and non-label electrochemical biosensor for the assay of myocardial miRNA based on cascade signal amplifications integrated by DNAzyme walker and hemin/G-quadruplex nanowires. The DNAzyme walker is activated by presence of target miRNAs to move along the electrode surface to cyclically cleave the substrate hairpins to release G-quadruplex segments, which further trigger the in situ formation of many hemin/G-quadruplex nanowires. The large amounts of hemin intercalated into the DNA nanowires subsequently generate drastically magnified electrochemical current signals for highly sensitive label-free assay of myocardial miRNAs down to 15.7 fM within dynamic range of 100 fM to 10 nM. Such a biosensor also has high selectivity and can monitor myocardial miRNAs in diluted serums at low levels, providing a sensitive and reliable platform for diagnosing infarct-associated cardiovascular diseases.

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靶标促进激活 DNAzyme walker 以原位组装 hemin/G-quadruplex 纳米线，从而实现超灵敏和无标记的电化学心肌 microRNA 检测。

心肌microRNA（miRNA）生物标志物浓度的升高与急性心肌梗死（AMI）的发病过程有关，而心肌miRNA生物标志物的灵敏定量对于AMI的早期诊断和治疗具有重要作用。为此，这项工作描述了一种超灵敏、无标记的电化学生物传感器，用于检测心肌 miRNA，该传感器基于 DNA 酶步行器和 hemin/G-quadruplex 纳米线集成的级联信号放大。目标 miRNA 的存在激活了 DNA 酶步行器，使其沿着电极表面移动，循环裂解底物发夹，释放出 G-四链段，进一步触发许多 hemin/G-quadruplex 纳米线的原位形成。插入 DNA 纳米线的大量海明随后会产生急剧放大的电化学电流信号，从而在 100 fM 至 10 nM 的动态范围内对低至 15.7 fM 的心肌 miRNA 进行高灵敏度的无标记检测。这种生物传感器还具有高选择性，可监测稀释血清中低水平的心肌 miRNA，为诊断梗死相关心血管疾病提供了一个灵敏可靠的平台。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.