Polychromatic Flow Cytometry to Identify Rare Aged Hematopoietic Stem Cell Subpopulations.

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2024-09-25 DOI:10.1007/7651_2024_570
Natalia Skinder, Christina Pitsillidou, Alessandra Roberto, Gerald de Haan
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Abstract

Flow cytometry enables the identification and characterization of markers present on the cell membrane as well as those that manifest intracellularly. With the increasing number of available reagents for targeting the markers of interest and evolving technology, it has become possible to detect an increasing number of markers expressed by single cells during one single analysis. This provides the possibility of investigating cell-to-cell patterns, variations, and correlations. Here, we describe a method to identify rare subpopulations of aged murine hematopoietic stem cell through polychromatic flow cytometry.

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多色流式细胞术识别罕见的老年造血干细胞亚群
流式细胞术可对细胞膜上的标记物以及细胞内的标记物进行鉴定和表征。随着用于靶向相关标记物的试剂越来越多,以及技术的不断发展,在一次分析中检测单个细胞表达的越来越多的标记物已成为可能。这为研究细胞间的模式、变化和相关性提供了可能。在这里,我们介绍了一种通过多色流式细胞术鉴定老龄鼠造血干细胞稀有亚群的方法。
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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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